
Scanning electron microscopy preparation protocol for …
2011年9月15日 · The lack of an established protocol for scanning electron microscopy (SEM) studies on stem cells differentiating into adipogenic lineage led us to develop a protocol for the preparation of differentiated adult bone marrow-derived mesenchymal stem cells (BMSC) for …
circ-Iqsec1 induces bone marrow-derived mesenchymal stem cell (BMSC …
Bone marrow-derived mesenchymal stem cells (BMSCs) are general progenitor cells of osteoblasts and adipocytes and they are characterized as a fundamental mediator for bone formation. The current research studied the molecular mechanisms underlying circRNA-regulated BMSC osteogenic differentiation.
Directed differentiation of BMSCs on structural/compositional …
2020年5月1日 · Here, the response of bone marrow stem cells (BMSCs) to electrospun gradient nanofibrous scaffolds was investigated to demonstrate their potential capabilities for interfacial tissue regeneration. The electrospun scaffolds showed gradient distribution of BMP-2/nanoHA contents and the fiber orientations gradually changed from random to align.
Human bone marrow mesenchymal stem cell-derived
2022年11月1日 · In detail, BMSC-EVs suppressed the aberrant proliferation, high collagen production capacity, and activation of fibrotic pathways in TGF-β-stimulated fibroblasts in vitro. Besides, in vivo, BMSC-EVs reduced cell infiltration, reduced fibrotic tissue in the shoulder capsule, and improved shoulder mobility. In addition, via exosomal small RNA ...
手把手教学——大鼠骨髓间充质干细胞(BMSCs)原代培养经验总结
BMSCs即为 骨髓间充质干细胞,是骨髓中基质细胞,是骨髓的支持结构,并可作为滋养层支持骨髓中造血干细胞的生长,因此也被称为骨髓基质干细胞。 作为干细胞的一种,它同样具有多向分化潜能,在一定的环境和细胞因子的作用下,可被诱导分化为骨细胞、神经元样细胞、脂肪细胞、软骨细胞、心肌细胞等。 其可被定义为具有多分化能力并且能自我更新复制的骨髓间质细胞。 其各项优点,使得它在临床干细胞移植治疗中显示出极大的应用前景。 目前己广泛使用在修复软 …
蛋白质组学探究骨髓干细胞来源的细胞外基质能优化神经细胞微环 …
此外,利用扫描电子显微镜 (SEM)和透射电子显微镜 (TEM)分析揭示细胞衍生的ECM和脱细胞神经的微结构 (纳米纤维的结构)。 结合蛋白质组学数据和细胞来源的ECM和脱细胞神经的结构特征,研究人员认为BMSC-ECM可能更接近于模仿天然神经ECM的组织特异性生化和结构属性。 图2 | 免疫染色3D、扫描电镜图和投射电镜图. A. ECMs结构与特点ecm的纤维连接蛋白 (白色)、层粘连蛋白 (红色)、I型胶原蛋白 (金色)、IV型胶原蛋白 (绿色)免疫染色3D图像,用Imaris软件分析,比例 …
SPEEK 促进 PEEK 表面 BMSCs 增殖和成骨分化的能力,Heliyon - X …
2024年8月18日 · 通过扫描电子显微镜 (SEM)、头状傅里叶变换红外 (FTIR) 光谱、热重分析 (TGA)、X 射线衍射 (XRD) 和差示扫描量热法 (DSC) 评价两种物种的物理化学性质。 然后,比较两组与纯聚醚醚酮 (PEEK) 在骨髓间充质干细胞 (BMSC) 表面接种后的增殖和成骨分化。 扫描电子显微镜 (SEM) 显示 PEEK 基材的表面可以是光滑的或粗糙的,并且粗糙度随着磺化的增加而增加。 FTIR 光谱显示 L-SPEEK 和 H-SPEEK 样品均含有磺酸。 TGA 和 XRD …
Osteoblasts and Bone Marrow Mesenchymal Stromal Cells Control ...
2010年2月8日 · The aim of this study was to investigate in vitro the role of human bone marrow–derived mesenchymal stromal cells (BMSC) and active osteoblasts in control of migration, lodgment, and proliferation of HSCs.
Biomaterials regulates BMSCs differentiation via mechanical ...
2024年2月1日 · It examines how the microenvironment of biomaterials affects the osteogenic differentiation of bone marrow mesenchymal stem cells through mechanical signals. The paper suggests design implications for biomaterials in bone regeneration and the construction of bone organoids, simulating in vivo bone tissue environments.
Metabolic programming determines the lineage-differentiation fate …
2019年11月14日 · Our data demonstrate that BMSC progenitors possess a distinct metabolic program and are poised to respond to exogenous metabolic cues that regulate their differentiation fate.