
Bromodeoxyuridine (BrdU) - BD Biosciences
Bromo-deoxyuridine (BrdU) is a thymidine analog that is used in cell proliferation studies. BrdU in culture is incorporated into the DNA during DNA synthesis. Cellular incorporation of BrdU can be detected by anti-BrdU specific antibodies following membrane permeabilization by flow cytometry or immunohistochemistry.
BrdU staining The immunofluorescent staining of incorporated bromodeoxyuridine (BrdU) and flow cytometric analysis provide a high-resolution technique to determine the frequency and nature of individual cells that have synthesized DNA.
FITC BrdU Flow Kit - BD Biosciences
BD FACSLyric™ Flow Cytometer Integrated with the BD FACSDuet™ Sample Preparation System
【罗工宝典第18式】BrdU的检测攻略 - 知乎
5-溴脱氧尿嘧啶核苷 (5-Bromo-2-deoxyUridine,BrdU),一种类似于DNA前体胸苷激酶的物质,在S期可替代 胸腺嘧啶核苷 选择性结合到细胞DNA中,当细胞处于DNA合成期(S期),而环境中同时又存在BrdU时,BrdU就可经活体注射或细胞培养插入复制的DNA双链中,且这种置换 ...
胸腺嘧啶核苷的类似物。当细胞处于细胞周期中的DNA合成期 (S 期) 时,BrdU� 以掺入到新合成的DNA 中。利用抗BrdU的单克隆抗体荧光染色,就可通过流式细胞术检测BrdU的含量,从而定量细胞群体的增
做实验 | 一文掌握 BrdU标记法检测细胞增殖 - 知乎
细胞经过固定和变性处理后,可用 免疫学方法 检测DNA中BrdU的含量 (如采用鼠抗BrdU单克隆抗体特异识别BrdU,再采用 辣根过氧化酶 标记的 山羊抗鼠IgG二抗 标记,最后用比色法或荧光的方法进行定量测定),从而判断细胞的增殖能力。
细胞增殖的流式检测(BrdU 法) - 哔哩哔哩
2024年11月7日 · 实验方法概述:在检测细胞增殖的多种方法中,直接测定DNA合成是一种敏感并高精度的分析方法;5-溴-脱氧尿嘧啶核苷 (Bromodeoxyuridine,BrdU),是一种胸腺嘧啶核苷的类似物,当细胞处于细胞周期中的DNA合成期 (S期)时,BrdU可以掺入到新合成的DNA中,利用 …
- [PDF]
Brdu 溶液使用说明
书 份 产品介绍: BrdU,英 文全称5-bromo-2′-deoxyuridine,中 文全称5- 溴-2’- 脱氧尿苷,是一种合成的胸苷溴化类似物,在 S 期可替代胸苷. 选择性插入细胞DNA。BrdU 通常和广泛用于测定DNA 合成和标记分裂细胞,最后用来研究诱导细胞增殖的信号通路和其他生理过程。BrdU通过体外细胞培养或体内注射的方式进行探针加载, 然后用抗BrdU 的抗. 体进行特异性检测。BrdU 标记后, 对组织或细胞进行固定和透化后, 需要额外的DNA 水解步骤( 有时也称DNA 变性),从而允许抗BrdU 的抗 …
The BD PharmingenTM BrdU In-Situ Detection Kit features our BD Retrievagen A antigen retrieval solution designed specifically to unmask antigenic sites, preserve tissue morphology, and enable simultaneous staining of other surface antigens in conjunction with BrdU.
Purified Mouse Anti- BrdU - BD Biosciences
Investigators can identify cycling cells in an asynchronous cell population and determine cell cycle kinetics by detecting incorporated BrdU. The 3D4 monoclonal antibody reacts with BrdU, but not other nucleotides, in single-stranded DNA.