
A novel method for oral delivery of drug compounds to the …
2007年4月4日 · BrdU was administered orally to neonatal SMNΔ7 SMA as well as non-SMA littermates for 3 days beginning at PND04 (n=3/genotype/drug). Genomic DNA from the kidney, forebrain and spinal cord (100 ng) was dot blotted onto a Nytran membrane and probed with a mouse monoclonal antibody directed against BrdU.
做实验 | 一文掌握 BrdU标记法检测细胞增殖 - 知乎
直接测定DNA合成是细胞增殖检测的最准确方法之一,是测定物质毒性、评估药物安全评价、细胞健康的基本方法,其中常用的方法有 BrdU标记法 。 >>> BrdU检测原理
Detection of BrdU-label Retaining Cells in the Lacrimal Gland ...
Double staining experiments for β-actin/BrdU and α-SMA/BrdU showed that label-retaining cells could be mobilized following lacrimal gland injury to differentiate into acinar, ductal and myoepithelial cells. In addition, a substantial amount of …
小鼠做了BrDU标记后影响别的免疫组化指标吗?比如SMA,E-cadhe…
理论上应该是不影响的,brdu是通过造模外源导进去的. 如果不确定,可以取平行切片做一下对比实验看效果. 你是做细胞凋亡么? 小鼠做了BrDU标记后影响别的免疫组化指标吗? 比如SMA,E-cadherin.谢谢.
【讨论】细胞增殖检测方法:EdU VS BrdU - 丁香通研选
2015年8月20日 · 与传统的免疫荧光染色(BrdU)检测方法相比,更简单,更快速,更准确。 EdU只有BrdU抗体大小的1/500,在细胞内更容易扩散,不需要严格的样品变性(酸解、热解、酶解)处理,有效地避免了样品损伤,有助于在组织、器官的整体水平上观测细胞增殖的真实情况,具有 ...
【罗工宝典第18式】BrdU的检测攻略 - 知乎 - 知乎专栏
5-溴脱氧尿嘧啶核苷 (5-Bromo-2-deoxyUridine,BrdU),一种类似于DNA前体胸苷激酶的物质,在S期可替代 胸腺嘧啶核苷 选择性结合到细胞DNA中,当细胞处于DNA合成期(S期),而环境中同时又存在BrdU时,BrdU就可经活体注射或细胞培养插入复制的DNA双链中,且这种置换 ...
小时以上持续的BrdU 掺入可以用于确定和分析处于DNA 合成活跃状态的细胞, 将其与静止期细胞区分开来,从而进行增殖细胞比例的分析。 针对具体的细胞类型
Characterization of the Early Proliferative Response of the Rodent ...
2012年10月12日 · Fluorescent BrdU labeling was quantified in cells within the urothelium, lamina propria (LP), muscularis propria (MP) and serosa. Cell location was confirmed with fluorescently co-labeled cytokeratin, vimentin or smooth muscle actin (SMA), to identify urothelial, interstitial and smooth muscle cells, respectively.
BrdU/EdU dual labeling to determine the cell-cycle dynamics
2018年2月14日 · Measuring the mean duration of synthesis-phase (T s) and of the total cell-cycle (T c) within progenitor cell populations can provide important insights into the biology governing these cells. Rather than a passive process that shows little variability across cellular contexts, the cell-cycle is instead highly regulated.
BrdU的检测攻略-优宁维 - univ-bio.com
5-溴脱氧尿嘧啶核苷(5-Bromo-2-deoxyUridine,BrdU),一种类似于DNA前体胸苷激酶的物质,在S期可替代胸腺嘧啶核苷选择性结合到细胞DNA中,当细胞处于DNA合成期(S期),而环境中同时又存在BrdU时,BrdU就可经活体注射或细胞培养插入复制的DNA双链中,且这种置换是 ...