C3A [HepG2/C3A, derivative of Hep G2 (ATCC HB-8065)] is an epithelial-like cell isolated from the liver of a White, 15-year-old male patient with hepatocellular carcinoma. This cell line was deposited by Baylor College of Medicine and used in cancer, toxicology, and …

Permanent human hepatocyte cell line and its use in a liver assist device (LAD). Mutations of the BRAF gene in human cancer. Signatures of mutation and selection in the cancer genome. A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

C3A cells are used in several BAL systems and the first phase III clinical trial of a C3A BAL has recently been completed (clinicaltrials.gov NCT00973817). HepaRG cells were also derived from a hepatocellular carcinoma and resemble hepatic progenitor cells in their capacity to differentiate into hepatocytes and cholangiocytes 8 .

C3A是Hep-G2的克隆性衍生物，具有较强的生长抑制、高白蛋白生成、高甲胎蛋白（AFP）生成和在缺乏葡萄糖的培养基中生长的能力。 基因表达:

2021年2月10日 · In this study, we developed an approach for genotoxicity testing with 21-day old spheroids formed from human hepatocellular carcinoma cells (HepG2/C3A) using the dynamic clinostat bioreactor system (CelVivo BAM/bioreactor) under controlled conditions.

C3A cells cultured with acute liver failure plasma showed mild inhibition of cell viabilities, reduction of albumin secretion, and increase of ammonia accumulation. Furthermore, CYP450 enzymes demonstrated various alterations on gene transcription, protein expression and enzyme activities.

2020年4月1日 · In this study, we investigated the effects of acute liver failure plasma on the growth and biological functions of C3A cells, especially on CYP450 enzymes. C3A cells were treated with fresh DMEM medium containing 10% FBS, fresh DMEM medium containing 10% normal plasma and acute liver failure plasma, respectively.

2020年1月1日 · Genetic restoration of urea cycle in C3A cells enhanced their capacity to synthesize albumin in standard culture conditions and under ammonia-induced stress. Flow cytometry analysis showed that the percentage of albumin-positive cells in the whole tested C3A population after lentiviral transductions raised 1.5-fold (C3A_AO vs. C3A ...

C3A, also referred to as HepG2/C3A, is a epithelial-like clonal variant derived from Hep G2, originating from the liver of a 15-year-old male with hepatocellular carcinoma. C3A cells exhibit robust contact inhibition of growth and demonstrate notable features such as high albumin and alpha-fetoprotein (AFP) production.

2008年2月15日 · Extrahepatic bioartificial liver devices should provide an intact urea cycle to detoxify ammonia. The C3A cell line, a subclone of the hepatoma-derived HepG2 cell line, is currently used in this context as it produces urea, and this has been assumed to be reflective of ammonia detoxification via a f …