CCR7 is a chemokine receptor for the chemokines CCL19 (CKbeta11, ELC, MIP3beta, Scya19, Exodus-3) and CCL21 (CKbeta9, SLC, MIP2beta, Scya21, Exodus-2). In addition to its significant role in the chemotaxis of lymphocytes, human CCR7 has also been recognized as a marker for a distinct subset of memory T cells, the central memory (TCM) population.

Jun 15, 2018 · Detection of CCR7 on Human Peripheral Blood Cells. Human whole blood was stained with Mouse anti-Human CCR7 APC-conjugated Monoclonal Antibody (Catalog # FAB197A) and Mouse anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) at 4 °C, room temperature (RT), or 37 °C.

Sep 1, 2023 · Upon exposure to the CCR7 ligand CCL19, dendritic cells (DCs) effectively internalize the receptor and ligand as part of the canonical GPCR desensitization response. We show that CCR7 internalization also acts as an effective sink for the chemoattractant, dynamically shaping the spatiotemporal distribution of the chemokine.

For many CCR stainings (like CCR7, eg) we stain at 37C for 10-15 minutes, then shift to RT and add the remaining antibodies for another 15 min. We rarely stain on ice anymore. Even for...

Moreover, we show that CCL19-S6 Dy649P1 serves as powerful reagent to monitor CCR7 internalization by time-lapse confocal video microscopy and to stain CCR7-positive primary human and mouse T cell sub-populations.

Oct 22, 2019 · Here, we uncover a function for an intracellular pool of the chemokine receptor CCR7 present in human dendritic cells and cellular model systems. We find that CCR7 signaling, initiated at the plasma membrane, is translocated by joint trafficking of β-arrestin and Src kinase to endomembrane-residing CCR7.

We demonstrate that CCR7 triggering alone is sufficient to recruit and activate ZAP70, a critical kinase for T cell activation, through Src kinase, whereas TCR CCR7 co-stimulation results in increased and prolonged ZAP70 kinase activity.

I am using PE and conjugates for staining of membrane extracellular proteins (CCR7, CXCR3, CD45RO), and I should stain for them after permeabilization, intracellular Mab addition and EdU...

Sep 9, 2024 · As discussed last week, human CCR7 staining is highly dependent on temperature during the incubation. Today we'll look at the behaviour of three different clones--G043H7, 3D12 and 2-L1-A--at different temperatures.

Mar 1, 2025 · In cancers, CCR7 has been revealed as a critical molecule in lymph node metastasis. Consequently, anti-CCR7 monoclonal antibodies (mAbs) have been developed as cancer therapeutic agents. In this study, we established an anti-mouse CCR7 (mCCR7) mAb, C 7 Mab-7 (rat IgG 1 , kappa) using the Cell-Based Immunization and Screening (CBIS) method.