Here we describe CD107a as a marker of NK cell functional activity using multi-parameter flow cytometry. CD107a is significantly upregulated on the surface of NK cells following stimulation with MHC devoid targets.

In this chapter, we detail the steps required to isolate peripheral blood mononuclear cells (PBMCs), coculture them with target tumor cell lines, and evaluate the cytotoxic immune function by means of flow cytometry evaluation of CD107a expression on the surface of NK cells.

Studies show that CD107a (LAMP-1) may be a marker for degranulation of NK and activated CD8+ T cells. In our study, the relationship between the expression of CD107a, cytokine secretion and cytotoxic activity in CD56+ NK, CD8+ T cells and …

2013年8月22日 · In particular, CD107a/LAMP-1 has been widely used as a marker for degranulating cytotoxic lymphocytes, because surface expression is induced upon degranulation. 22-24 CD107a is a highly glycosylated protein comprising a 40-kDa backbone with predicted 17 N -glycosylation and 9 O -glycosylation sites.

2004年11月1日 · Recently, lysosomal-associated membrane protein-1 (LAMP-1 or CD107a) has been described as a marker of CD8 + T-cell degranulation following stimulation. Here we describe CD107a as a marker of NK cell functional activity using multi-parameter flow cytometry.

CD107a glycoproteins line the luminal surface of resting T cells. Upon activation, lytic granules get localized to the site of interaction with the target cell and merge with the plasma membrane. During this process, granzymes and perforin get exocytosed and …

2013年6月6日 · Lysosome-associated membrane protein (LAMP) 1/CD107a is used as a marker for NK-cell degranulation, but its role in NK-cell biology is unknown. We show that LAMP1 silencing causes inhibition of NK-cell cytotoxicity, as LAMP1 RNA interference (RNAi) cells fail to deliver granzyme B to target cells.

2004年1月1日 · High-level expression of CD107a and b on the cell surface of activated T cells requires degranulation, because degranulation inhibitors, such as colchicine, dramatically reduce cell-surface expression of CD107a and b (Betts et al., 2003).

Recently, lysosomal-associated membrane protein-1 (LAMP-1 or CD107a) has been described as a marker of CD8+ T-cell degranulation following stimulation. Here we describe CD107a as a marker of NK...

CD107a is a marker of degranulation of cytotoxic NK and CD8 + T-cells . The present study demonstrates a decreased expression of CD107a on CD8 + T-cells SLE-patients as compared to HC. The decreased proportion of CD107a + CD8 + T-cells was especially found in SLE-patients without lupus nephritis.