Genetic Inactivation of CD33 in Hematopoietic Stem Cells ... - Cell …
We show here that CD33 knockout (KO) HSPC can generate functional hematopoiesis that is resistant to CD33-targeted AML therapy in two relevant preclinical models and provide …
CRISPR/Cas9 editing of NKG2A improves the efficacy of primary CD33 …
2024年9月30日 · We generate CD33-specific, AML-targeted CAR-NK cells (CAR33) combined with CRISPR/Cas9-based gene disruption of the NKG2A-encoding KLRC1 gene. Using single …
Genetic Inactivation of CD33 in Hematopoietic Stem Cells to
2018年5月31日 · Autologous CD33 KO HSPC transplantation in rhesus macaques demonstrated long-term multilineage engraftment of gene-edited cells with normal myeloid function. CD33 …
Genetic Inactivation of CD33 in Hematopoietic Stem Cells to …
We show here that CD33 knockout (KO) HSPC can generate functional hematopoiesis that is resistant to CD33-targeted AML therapy in two relevant preclinical models and provide …
CRISPR/Cas9 editing of NKG2A improves the efficacy of primary CD33 …
2024年9月30日 · We generate CD33-specific, AML-targeted CAR-NK cells (CAR33) combined with CRISPR/Cas9-based gene disruption of the NKG2A-encoding KLRC1 gene. Using single …
Targeting the membrane-proximal C2-set domain of CD33
2024年9月19日 · Current CD33-targeted immunotherapies typically recognize the membrane-distal V-set domain of CD33. Here, we show that decreasing the distance between T cell and …
Optimization of CRISPR-Cas9 knock-out of CD33 in human …
2019年5月1日 · IVT gRNA demonstrated better knock-out (KO) efficiency than both synRNAs (CD33 surface expression baseline 97.65%; IVT gRNA 0.87-1.33%; synRNA-1 2.15-2.25%, …
Development of a gene edited next-generation ... - ScienceDirect
2023年12月14日 · CD33-KO cells were diluted with unedited cells to generate an editing titration curve from 10% to 90% editing frequency, with 90% being CD33-KO cells alone. Following 9 …
CD33‐directed immunotherapy with third‐generation chimeric …
2021年11月11日 · The human CD33-positive myeloid cell line MV4-11 was used to test the knockout (KO) efficiency of the six gRNAs resulting in functional loss of CD33. Lentiviruses …
Autologous CD33 KO HSPC transplan-tation in rhesus macaques demonstrated long-term multilineage engraftment of gene-edited cells with normal myeloid function. CD33-deficient …