Results: ELISpot screening readily identified HBV-specific CD4 and CD8 T cell responses in acute resolving infection compared with more limited reactivity in chronic infection. Applying more sensitive assays revealed higher frequencies of functional HBV-specific CD4 T cells, but not CD8 T cells, in functional cure compared to chronic infection.

In this review, we summarize what is known about CD4 + T cells in chronic HBV infection and discuss the importance and opportunities of including CD4 + T cells in T cell-directed immunotherapeutic strategies to cure chronic HBV. Keywords: CD4 + T cells, hepatitis B virus, immunotherapy. 1. Introduction

TNF-α producing cells are the dominant population of hepatitis B virus (HBV)-specific CD4 T cells in patients with chronic HBV infection. This population of cells might contribute to the aggravation of liver damage in patients with a hepatitis B flare.

2020年6月23日 · 体外刺激hbv特异性cd4 t淋巴细胞上ox40分子（抗原递呈细胞上共刺激分子ox40l的配体）、同时阻断pd-1分子，能显著提升hbv特异性 ifnγ+及il-21+ cd4 t淋巴细胞的数量，这提示给予适当的天然免疫信号刺激，能有效诱导hbv特异性cd4 t淋巴细胞的进一步分化[31]。

2023年3月13日 · In this study, using a series of samples from a longitudinal cohort of CHB patients with NA discontinuation and peptides spanning the whole HBV genome, we found boosted HBV Pol-specific CD4 + T cells at baseline withdrawal; Core-specific and Pol-specific CD4 + T-cell responses are related to sustained viral control after long-term treatment ...

2021年5月6日 · In this review, we summarize what is known about CD4 + T cells in chronic HBV infection and discuss the importance and opportunities of including CD4 + T cells in T cell-directed immunotherapeutic strategies to cure chronic HBV.

从产生hbv特异性tnf-α的cd4 t细胞分化为产生hbv特异性ifn-γ的cd4 t细胞可能有利于hbv病毒的清除。 在本研究中，作者首先分析了68例 丙氨酸氨基转移酶 （ALT）低于正常上限（ULN）2倍的慢性HBV感染患者的HBV特异性CD4 T细胞的IFN-γ /TNF-α功能谱。

2019年12月31日 · 在ALT <2xULN的患者中，在病毒清除水平升高的患者中，产生HBV特异性IFN-γ的CD4 T细胞的频率和优势依次增加：HBV e抗原（HBeAg）阳性，HBeAg阴性和HBV表面抗原（HBsAg）阴性。 在患有乙型肝炎的患者中，产生HBV核心的特异性TNF-α的CD4 T细胞的频率与患者的ALT和总胆红素水平呈正相关，并且这些细胞的频率与肝损害的严重程度相关。 产生HBV S特异性IFN-γ的CD4T细胞的频率和优势与爆发期间HBsAg的减少呈正相关。 结论. 在患有慢 …

2024年9月1日 · CD40 stimulation in CD4-depleted AAV-HBV mice resulted in the clearance of HBsAg and HBeAg, along with a reduction in liver HBV mRNA, contrasting with CD4-competent counterparts. CD8 + T cells were indispensable for CD40-mediated HBV control, determined by HBV persistence following their depletion.

Our data indicate that higher frequencies of functional HBV-specific CD4 T cell responses are a key component of the immune signature associated with functional cure, and that direct ex vivo analysis of HBV-specific CD4 T cells, as demonstrated here, is a promising approach for identifying the molecular correlates of CD4-mediated immune protection.