CD69 is a lymphoid activation antigen whose rapid expression (< or = 2 h postactivation) makes it amenable for the early detection of T-cell activation and for subset activation analyses. In the present study we evaluated the utility of flow cytometric detection of CD69 expression by …

2001年1月1日 · The expression of activation antigens, namely CD25, CD69, CD71, and HLA-DR on T cells from 15 healthy individuals stimulated with different mitogens and specific antigens was evaluated by immunofluorescence assay and flow cytometric analysis and compared with cell proliferation as a function of [3H] …

2024年11月4日 · Subsequently, T cells were analyzed by FACS and ELISA. Using CD69 as the marker of activated Jurkat T cells, we identified 27 cognate TCRs targeting epitopes of SARS-CoV-2 (Fig. 4A,...

2018年9月5日 · CD69 over-expression stimulated higher levels of IL-10 and c-Maf expression, which was compromised by silencing of STAT3 or STAT5.

We confirmed that the expression of Tmem119/Sall1 and Ccr2/CD69 differed in FACS-sorted microglia- and monocyte/macrophage-enriched cell populations, respectively. These isolated cells had maximally preserved biologic characteristics after ICH, including inflammatory responses, phagocytosis, and dynamic polarization.

Specifically, whole-blood samples were incubated with PHA for 18 to 24 h, and the degree of T-lymphocyte activation was determined qualitatively by the proportion of activated CD4 and CD8 T lymphocytes expressing CD69 (percent CD69) and semiquantitatively by measuring the level of CD69 expression (anti-CD69 ABC) on each activated T-lymphocyte ...

2015年6月15日 · T cells and NK cells upregulate surface marker CD69 upon activation. CD69 is one of the early activation markers in T cell stimulation/ activation and thus the stimulation or activation state of the cells can be easily measured by analyzing the number/ percent of T cells expressing CD69.

CD69 is a lymphoid activation antigen whose rapid expression (< or = 2 h postactivation) makes it amenable for the early detection of T-cell activation and for subset activation analyses. In the present study we evaluated the utility of flow ...

2007年1月1日 · Healthy volunteers showed 17-fold increases in T-cell CD69 Ab bound per cell (ABC) with PHA stimulation compared with the baseline. SEB was also an effective T-cell activating agent, increasing CD69 ABC by 5-fold, comparable with …

Together, our data suggest that CD69 is a useful marker for cytotoxic activity of NK cells, whereas proliferative potential is indicated by CD25 expression. These findings should help optimizing the ex-vivo generation of large numbers of cytotoxic effector cells for immunotherapy.