CFU-GM represents the progeny of a single CD34+ cell committed to the myeloid lineage. You might find these chapters and articles relevant to this topic.

CFU-GEMM (Colony-forming unit − granulocyte, erythroid, macrophage, megakaryocyte) • A colony containing both erythroid (hemoglobinized) cells and 20 or more non-erythroid (not hemoglobinized) cells, i.e. granulocytes, macrophages, and/or megakaryocytes • Typically has erythroid cells in the center and non-erythroid cells

The colony-forming unit (CFU) assay is one of the most widely used assays for hematopoietic stem and progenitor cells (HSPCs). CFU assays allow measurement of the proliferation and differentiation ability of individual cells within a sample.

The CFU-GM assay is used in a screening mode to predict chemical induced myelosuppression, one of the haematological parameters to be considered for the assessment of the haematotoxic potential of chemical compounds of various kinds.

Higher post-thaw CFU-GM dose showed an increased benefit for neutrophil and platelet engraftment (p < 0.01). Post-thaw CD34+ cell dose and CFU-GM dose were strongly correlated (r = 0.78). However, CFU-GM dose showed additional benefit for patients receiving the lowest quartile of CD34+ dose.

2019年6月14日 · The CFU-GEMM are progenitors that can form colonies, in semisolid culture medium, comprising mixtures of granulocytes, erythroblasts, megakaryocytes, and macrophages [16]. Clonogenic assays are the most directly quantitative means of measuring human hematopoietic progenitor cells in vitro [16].

The colony forming unit (CFU) is a measure of viable colonogenic cell numbers in CFU/mL. These are an indication of the number of cells that remain viable enough to proliferate and form small colonies. Isolated hMSCs were plated in a 6-well cell culture plate along with …

The colony forming unit (CFU) cell assay, or CFC assay, is used to study the proliferation and differentiation of hematopoietic progenitors by their ability to form colonies in a semisolid medium such as methylcellulose or agar.

The colony-forming unit-granulocyte-macrophage (CFU-GM) assay is used commonly to assess adequacy of progenitor number in bone marrow transplantation. The assay is poorly standardized, resulting in variability of results between and within laboratories.

Wide acceptance of the colony-forming-unit (CFU) assay as a reliable potency test for stem cell products is hindered by poor inter-laboratory reproducibility. The goal of this study was to ascertain current laboratory practices for performing the CFU assay to identify practices that could be standardized to improve overall reproducibility.