2014年1月7日 · Here, we investigated the function of CURLY LEAF (CLF), a Polycomb-group (PcG) gene responsible for histone3 lysine 27 trimethylation (H3K27me3), in somatic and meiotic HR in Arabidopsis thaliana.

在减数分裂周期中，由 Spo11蛋白 介导产生的 DNA双链断裂 （DNA double-strand breaks，DSBs）引发的同源重组对于基因组稳定性的维持非常关键 【1】。 DSBs形成过程是受到调控的，同时也与大范围的染色体结构形成相关，但是一直以来调节和控制DNA双链断裂的蛋白质装配体组织方式还不得而知。

This new method offers researchers a versatile, fast, low-cost, titratable method for inducing DSBs in any electroporatable cell line, allowing for a systematic exploration of DSB repair in a range of cell and tissue types.

In Arabidopsis, the H3K27 methyltransferase CURLY LEAF (CLF), a homolog of the polycomb-group (PcG) protein EZH2 in mammals, is associated with the epigenetic regulation of somatic and meiotic HR repair .

Nuclease-induced double strand breaks (DSBs) can be repaired by nonhomologous end joining (NHEJ) or homology-directed repair (HDR) pathways. Imprecise NHEJ mediated repair can produce insertion and/or deletion mutations of variable length at the site of the DSB.

2020年11月2日 · sBLISS (in-suspension breaks labeling in situ and sequencing) is a versatile and widely applicable method for identification of endogenous and induced DNA double-strand breaks (DSBs) in...

2020年2月20日 · In this review, we present these tools, discuss their pros and cons and illustrate their contribution to our current understanding of the DDR. DNA double-strand breaks (DSBs) are the most cytotoxic DNA lesions. Their detection, signaling, and repair require a comprehensive cellular response collectively known as the DNA damage response (DDR).

ACRs are mainly responsible for transcriptional regulation of several homologous recombination (HR) repair genes in plants under genotoxic stress. The HR-based repair of DNA damage has been considered as the most error-free mechanism of repair and represents one of the essential sources of genetic diversity and new allelic combinations in plants.

电离辐射（IR-）诱导的DNA双链断裂（DSB）被认为是有害的DNA损伤，对基因组稳定性构成严重威胁。 主要的DNA修复途径，包括经典的非同源末端连接，同源重组，单链退火和替代末端连接，在抵抗和诱导IR诱导的DSB确保基因组完整性方面起着关键作用。 如果未正确修复IR诱导的DNA DSB，则残留或未正确修复的DSB可能导致与某些人类疾病相关的基因组不稳定。 尽管在研究IR诱导的DNA DSB修复的主要机制方面已作了许多努力，但仍不清楚是什么决定IR诱导的DNA …

我们可以预先设定控制定律 u=-2x_{1}-x_{2} ，通过CLF函数 V=\frac{5}{8}x_{1}^{2}+\frac{1}{2}x_{1}x_{2}+\frac{1}{2}x_{2}^{2} （及其对时间的导数）可以验证，我们设计的控制律是能够使得系统轨迹到达平衡点的。