In mutants clf 28 and lhp 1, failure to deposit H3K27me3 in active genes upon cold treatment implies that the CLF is potentially involved in cold-induced deposition of H3K27me3, with assistance from LHP1. Failure to deposit H3K4me3 during cold treatment in atx 1-2 suggests a regulatory role of ATX1 in the deposition of H3K4me3.

2022年9月12日 · Here, we report that combining mutations for CURLY LEAF (CLF) and LIKE HETEROCHROMATIN PROTEIN1 (LHP1), two Arabidopsis PcG proteins, results in deregulation of expression of active genes that are targeted by PcG proteins or enriched in associated chromatin marks.

2006年3月20日 · The vernalization-induced epigenetic silencing of FLC is associated with histone deacetylation and H3K27me2 and H3K9me2 methylation mediated by VRN/VIN proteins. We have analyzed whether different histone methyltransferases and the chromodomain protein LIKE HETEROCHROMATIN PROTEIN (LHP)1 might play a role in vernalization.

2024年1月15日 · 该研究通过re-ChIP和ChIP-seq等方法，联合开放染色体质数据，从全基因组层面鉴定拟南芥低温诱导产生的二价组蛋白修饰，并利用 clf28、lhp1和atx1-2等突变体，联合解析低温诱导二价组蛋白修饰染色质的产生及核小体密度降低现象的分子机制。 组蛋白修饰对真核生物基因表达起重要调控作用。

2016年7月13日 · Here, we show that LHP1 is responsible for the spreading of H3K27me3 towards the 3’ end of the gene body. We also identified a subset of LHP1-activated genes and demonstrated that LHP1 shapes local chromatin topology in order to …

2019年8月22日 · In the current study we show that the PRC1 protein LHP1 is required for the repression of the MYC2 branch of jasmonic acid (JA)/ethylene (ET) pathway of immunity. Loss of LHP1 induces the reduction in H3K27me3 levels in the gene bodies of ANAC019 and ANAC055, as well as some of their targets, leading to their transcriptional upregulation.

In mutants clf28 and lhp1, failure to deposit H3K27me3 in active genes upon cold treatment implies that the CLF is potentially involved in cold-induced deposition of H3K27me3, with assistance from LHP1. Failure to deposit H3K4me3 during cold treatment in atx1-2 suggests a regulatory role of ATX1 in the deposition of H3K4me3.

( J) Venn diagram showing differential H3K27me3 deposition in lhp1 and clf. A high overlapping of Hypo and Hyper-methylated genes can be observed between lhp1 and clf, as indicated in the red boxes.

同源重组 (Homologous recombination,HR)是指同源染色体发生交换.在减数分裂过程中,同源重组对染色体的正确分离是必须的,同时也是生物进化及农作物育种的基础.体细胞中,同源重组是修复DNA损伤的一种重要方式.拟南芥CLF是组蛋白H3K27 (第27位赖氨酸)甲基转移酶,LHPl是 ...

Loss of CLF, LHP1 or EMF1 function rescues FT silencing and late flowering in the nf-yb2 nf-yb3 and nf-yc3 nf-yc4 nf-yc9 mutants. (a) Phenotypes of long-day-grown mutants. Scale bars: 1.0 cm....