The presence of CMV is indicated by its typical cytopathic effect (CPE), characterized by foci of flat, swollen cells (2, 3). Notably, the time to the development of CPE has been correlated directly with the titer of CMV present in the sample.

1997年7月19日 · We examined the following hypothesis: CMV, present in monocytes recruited to sites of vascular injury, is activated by endothelial cell (EC) or smooth muscle cell (SMC) contact and by oxidized low-density lipoproteins (oxLDLs). The CMV major immediate-early promoter (MIEP) controls immediate-early (IE) gene expression, and thereby viral ...

Congenital CMV disease manifested as cytomegalic inclusion disease and characterized by jaundice, petechial rash, microcephaly, hepatosplenomegaly, chorioretinitis, cerebral calcifications, hearing defects, lethargy, and seizures. Sensorineural hearing defect is …

2002年2月1日 · In fibroblast cell cultures CMV replicates to produce cytopathic effect (CPE) after 2–4 weeks incubation. This slow evolution of CPE is typical of the Betaherpesvirinae and represents a major impediment to studying CMV replication in the laboratory.

Although herpes simplex virus (HSV) may produce easily visible CPE within the first 24 h of incubation, most viruses demonstrate CPE only after 5 to 10 days of incubation, with some, such as cytomegalovirus (CMV), averaging 10 to 30 days for CPE production (Table 2).

Clinical samples were also inoculated into three MRC-5 or MRHF cell cultures which were observed for 30 days for the appearance of a cytopathic effect (CPE). Of 157 CMV-positive samples, 92 (59%) were identified by centrifugation-enhanced EA (CE-EA) and 131 (83%) produced a CPE.

CMV-Cre: This transgene expresses Cre recombinase under the control of a human cytomegalovirus (CMV) promoter, active in most cells and tissues. Homozygous transgenic mice are viable and fertile. The transgene appears to be X-linked since transgene transmission through males is restricted to female offspring.

The association of cytomegalovirus (CMV) with a plethora of diseases and the recent development of potent anti-CMV compounds warrants the need for accurate verification of prior exposure to CMV as well as the identification of infectious virus in clinical specimens.

A method of pre-cytopathological effects (CPE) detection in shell vial cultures using monoclonal antibodies (mAb) was evaluated for detection of cytomegalovirus (CMV) in pediatric specimens. The diagnostic value of antibody detection by enzyme immunoassay (EIA) was also evaluated. CMV was isolated f …

A pre-cytopathic effect (CPE) monoclonal antibody reagent (Syva Co., Palo Alto, Calif.) was evaluated in four laboratories for the rapid detection of cytomegalovirus (CMV) in shell vial cell cultures at 16 to 24 h and 40 to 48 h postinoculation. Results were compared with …