Quantitative analyses for effects of neddylation on CRL2 VHL …
Our results demonstrate that neddylation plays a significant role in activating CRL2, and the cellular activity of CRL2 VHL is better reflected by the degradation of CODD than that of …
Mechanism of degrader-targeted protein ubiquitinability
2024年10月11日 · Here, we show cryo-EM structures of the VHL Cullin 2 RING E3 ligase with the degrader MZ1 directing target protein Brd4 BD2 toward UBE2R1-ubiquitin, and Lys 456 at …
Von Hippel-Lindau (VHL) small-molecule inhibitor binding …
Von Hippel-Lindau (VHL) disease is characterized by frequent mutation of VHL protein, a tumor suppressor that functions as the substrate recognition subunit of a Cullin2 RING E3 ligase …
The structure and regulation of Cullin 2 based E3 ubiquitin …
2016年5月23日 · The most well-known CRL2 substrate recognition receptor is the tumor suppressor protein VHL that is mutated in von Hippel–Lindau (VHL) syndrome, a rare …
Structural basis of Cullin 2 RING E3 ligase regulation by the COP9 ...
2019年8月23日 · Mutations in the interface between VHL, ELOB and ELOC can deactivate CRL2 leading to an accumulation of HIF-1α, which can in turn drive tumorigenesis through the over …
Expression and purification of functional recombinant CUL2•RBX1 …
2021年5月27日 · In our in vitro assays, the recombinant CUL2•RBX1 purified from either expression system can catalyze the conjugation of NEDD8 to CUL2 and can bind VHL•EloB/C …
neddylation 对 CRL2VHL 底物泛素化和降解影响的定量分 …
2021年8月30日 · 通过催化关键调控蛋白的泛素化，cullin-RING 泛素连接酶 (CRL) 发挥着重要的生物学作用，其活性受多种机制控制，包括 neddylation、NEDD8 与 cullin 的结合。 在 …
Crystal Structure of the Cul2-Rbx1-EloBC-VHL Ubiquitin
2017年6月6日 · Here, we present the first crystal structure of the CRL2 VHL complex to 3.9 Å resolution, revealing for the first time the full-length Cul2 interacting with Rbx1, which provides …
Differential PROTAC substrate specificity dictated by orientation of ...
2019年1月10日 · Endogenously, VHL is the substrate-binding element of the Cullin 2 RING E3 ligase (CRL2 VHL complex), constitutively targeting Hypoxia-inducible factor 1-alpha (HIF-1α) …
K29/K48-branched ubiquitin chains TRIP the alarm fueling neo …
2021年4月1日 · The authors propose that the ubiquitin chain formation mediated by CRL2 VHL and TRIP12 is cooperative and bi-directional, whereby TRIP12 is dependent on CRL2 VHL to …