2009年7月16日 · Cholera toxin subunit B (CTB) is a highly sensitive retrograde neuroanatomical tracer. With the new availability of fluorescent Alexa Fluor (AF) conjugates of CTB, multiple...

逆行示踪技术（retrograde tracing）也称为逆行标记技术，是神经科学中的一种常用研究方法，1967年Fink等将神经纤维变性研究与银浸渍染色技术两者结合应用后开创了逆行示踪技术的首次尝试，并取得了开创性的结果，…

Recently, Alexa Fluor (AF) conjugates of CTB have been made available, which now allows multiple tracing with CTB. In this study, we examined the efficacy of these new AF-CTB conjugates when injected into the brain, and compared the results to our previous experiences using fluorescent 3k dextran amines.

We report an improved immunohistochemical protocol for revealing anterograde axonal transport of the subunit B of cholera toxin (CTB) which stains axons and terminals in great detail, so that single axons can be followed over long distances and their arbors reconstructed in their entirety.

Cholera toxin subunit B (CTB) is a highly sensitive retrograde neuroanatomical tracer. With the new availability of fluorescent Alexa Fluor (AF) conjugates of CTB, multiple neuroanatomical connections can be reliably studied and compared in the same animal. Here we provide a protocol that describes …

CTb is a very sensitive retrograde neuro-anatomical tracer which can be detected either by immunochemical methods, or by the use of CTb conjugates such as CTb-HRP and CTb-FITC or CTb-TRITC, which can be visualized after histochemical detection and by fluorescence microscopy, respectively.

2019年8月26日 · Cholera toxin subunit B (CTb) was introduced as a retrograde tracer in 1977 (Stoeckel et al., 1977; Vercelli et al., 2000). Trojanowski et al. (1981, 1982) then sought to improve the sensitivity of HRP using CTb-HRP conjugates, and found that when the number and detail of labeled neurons was compared, CTb-HRP significantly out-performed free HRP.

2015年12月7日 · The cholera toxin B subunit (CTB) binds to cellular surfaces via its receptor monosialoanglioside (GM1) [11]. As a conventional bacterial toxin for neuronal tracing, CTB enters the axons or dendrites by active uptake via nerve terminals, or through severed axons [12].

2009年7月21日 · Recently, Alexa Fluor (AF) conjugates of CTB have been made available, which now allows multiple tracing with CTB. In this study, we examined the efficacy of these new AF-CTB conjugates when injected into the brain, and compared the results to our previous experiences using fluorescent 3k dextran amines.

2022年5月10日 · To identify tracer dye distribution in sensory neurons within ganglia and sensory terminals within the spinal cord and medulla, 4-7 days after surgery, fix animals by transcardial perfuse fixation, then remove tissues of interest for further study.