Cas12a (CRISPR-associated protein 12a, previously known as Cpf1) is an RNA-guided endonuclease-exonuclease that forms an essential component of the CRISPR systems found …

DETECTR技术使用V型酶（如Cas12a）切割单链DNA序列，分为三个阶段：（1）将Cas12a蛋白和靶crRNA整合进DNA报告探针中，一旦crRNA通过Cas12a蛋白识别其靶序列，Cas12a蛋白 …

Cas12a 又称为 Cpf1 ，Class 2类 CRISPR系统 ，与Cas9一样通过识别特异性 PAM序列 行使 核酸内切酶 功能从而产生DNA双链断裂。 与Cas9系统在crRNA加工、PAM识别机制、切割方式等 …

Sep 25, 2023 · Cas12a. Cas12a(又称Cpf1)是一类由单个crRNA介导的核酸内切酶，能特异性识别并剪切带PAM(5-TTTN-3'或5'-TTN-3)的双链DNA(dsDNA)靶标，使DNA双链断裂并生成 黏性 …

Class II type V CRISPR-Cas12a is a new RNA guided endonuclease that has been recently harnessed as an alternative genome editing tool, which is emerging as a powerful molecular …

Cas12a使用RuvC核酸内切酶结构域对DNA实现远离PAM交错切割，同时将PAM远端切割产物释放，产生长度为4~5nt突出的黏性末端缺口。 随后，细胞启动修复机制以修复损伤缺口。

Feb 1, 2020 · In this review we have sought to offer a condensed overview of the functionality of CRISPR-Cas12a, discussing the structural and functional features of the different stages of …

Oct 19, 2023 · 【干货分享】CRISPR/Cas系统原理及Cas9与Cas12a区别，从多方面入手，详细介绍CRISPR/Cas系统中Cas9蛋白和Cas12a蛋白的区别

Mar 28, 2025 · 此外，Cas12a的PAM序列要求相对宽松（TTTN），使其在基因组中的靶向范围更广，能够覆盖更多难以被Cas9识别的位点。因此，Cas12a在基础研究、疾病模型构建以及潜 …

Jul 13, 2020 · We selected Acidaminococcus Cas12a (AsCas12a) as an optimal starting point for engineering given its high gene editing activity in prior work 14.