Recent computational efforts to identify new CRISPR systems uncovered a novel type of RNA targeting enzyme, Cas13. The diverse Cas13 family contains at least four known subtypes, …

CRISPR-Cas13系统属于 Type VI家族，包含多个家族，其中Type A、Type B、Type D、Type X和Type Y家族的功能被鉴定出来（图1.2）。 Cas13蛋白都是由多结构域组成的单一蛋白， …

2022年10月11日 · By mining bulk metagenomic data (> 10 TB) from various environments, we identified hundreds of orthologs of known and novel Cas13 systems in this study, the latter of …

2023年9月18日 · CRISPR-Cas13 系统属于Type VI家族，包括Cas13a、b、c、d等多个亚型。Cas13蛋白都是由多结构域组成的单一蛋白，具有识别crRNA、切割RNA、甚至是切割pre …

2021年5月3日 · By analyzing metagenomic terabase datasets, we identified two compact families (775 to 803 amino acids (aa)) of CRISPR–Cas ribonucleases from hypersaline samples, …

2024年5月3日 · This Review focuses on CRISPR–Cas type VI Cas13 systems that use single-subunit RNA-guided Cas endonucleases for targeting and subsequent degradation of foreign …

This study highlighted the latest progress in RNA editing targeted by the CRISPR-Cas13 family, and discussed the application of Cas13 in basic research, nucleic acid diagnosis, nucleic acid …

2017年10月25日 · We assayed a subset of the family of Cas13 enzymes for RNA knockdown activity in mammalian cells and identified the Cas13b ortholog from Prevotella sp. P5-125 …

The Cas13 protein family contains two subtypes: (1) Cas13a protein from Leptotrichia shahii bacterium (LshCas13a), which is formally known as C2c2 and belongs to type VI, and (2) …

2020年3月1日 · CRISPR-Cas13 is the system in the CRISPR-Cas family that targets only ssRNA so far, which lays a foundation for RNA targeting and RNA editing. It has recently been …