2016年4月1日 · In this review, we describe how CRISPR/Cas9 works and how it can be applied in plants, especially crop plants. We also discuss the pitfalls of this technique and its future development for crop genetic improvement.

2025年1月1日 · CRISPR/Cas9 technology has dramatically improved crop production over the past ten years despite its limitations. The CRISPR/Cas9 approach can potentially alter the future generation of agriculture by exploring the capabilities of the chosen crop species, enhancing its productivity, stress tolerance, and nutritional efficiency.

2025年3月7日 · Cas9 orthologues and variants. The type II CRISPR systems are categorized into subtypes II-A, II-B and II-C 18 (Table 1).The subtype II-A SpCas9 was a Cas nuclease used initial in plant genome ...

The CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated protein9) system is an RNA-guided genome editing tool that consists of a Cas9 nuclease and a single-guide RNA (sgRNA). By base-pairing with a DNA target sequence, the sgRNA enables Cas9 to recognize and cut …

The CRISPR/Cas9 system has become the most used and versatile technology in crop breeding and functional genomics. With the incomparable capability to modulate genes, it helped create numerous crop varieties with desired agronomic performances.

2023年3月1日 · CRISPR/Cas9, with its simplicity, efficiency, inexpensiveness and elegant way of genomic editing, offers a wide possibility to plant breeders for crop improvement. This review highlights the past accomplishment, current research and future prospects of CRISPR technology in crop improvement.

In this review, recent progress in utilizing CRISPR/Cas9-mediated gene editing in plants to enhance certain traits in beneficial crops, including rice, soybean, and oilseed rape, is discussed. In addition, novel methods of applying the CRISPR/Cas9 system in live cell imaging are also extensively reviewed.

2021年4月19日 · This review summarizes the current application of CRISPR/Cas9 technology in crop quality improvement. It includes the modulation in appearance, palatability, nutritional components and other preferred traits of various crops.

2024年12月2日 · This review summarized the delivery patterns, mutation detection, and application of the CRISPR-Cas9 system in tropical crop breeding, discussed the existing problems, and addressed prospects for future applications in this field, providing references to relevant studies.

Advancements in Genome editing technologies have revolutionized the fields of functional genomics and crop improvement. CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat)-Cas9 is a multipurpose technology for genetic engineering that relies on the complementarity of the guideRNA (gRNA) to a specific sequence and the Cas9 ...