ccdB基因位于大肠杆菌的 F质粒 上，是被ccd操纵子编码的毒性-抗毒性系统的一部分，确保含F质粒的细胞分裂后F质粒仍存在（分裂后含F质粒的细胞才能存活）。 ccdB编码的毒性蛋白 …

The CcdA/CcdB Type II Toxin-antitoxin system is one example of the bacterial toxin-antitoxin (TA) systems that encode two proteins, one a potent inhibitor of cell proliferation (toxin) and the …

2017年12月8日 · The ccdB gene, located on the F sex factor plasmid of E. coli, is part of a toxin-antitoxin system encoded by the ccd operon, which is responsible for plasmid maintenance …

In this study, we used the CcdB toxin as a selection marker to improve Cpf1-based plasmids designed for genome-editing in cyanobacteria. Our results demonstrate that this selection …

Gateway 的核心之一就是利用 ccdB 的毒性，从而达到negative selection. ccdB是一种DNA gyrase， 其工作原理如下图： 它能让DNA的超螺旋结构变成逆向螺旋，因此对 E.coli 具有毒性 …

2022年1月2日 · The first commercial application of a toxin–antitoxin system used the ccdB toxic gene to generate a cloning vector that enables positive selection 219,220.

Cell killing by CcdB is accompanied by filamentation, defects in chromosome and plasmid segregation, defects in cell division, formation of anucleate cells, decreased DNA synthesis …

CcdB, the toxin from the ccd system on the Escherichia coli F plasmid, acts as a gyrase poison [1]. The ccd operon of the F plasmid encodes CcdB, a toxin targeting the essential gyrase of …

2023年5月4日 · ccdB基因位于大肠杆菌 F性因子质粒 上，是 ccd操纵子 编码的毒素-抗毒素系统的一部分，负责细胞分裂过程中质粒的维持。 ccdB编码毒性蛋白( CcdB )，它作为DNA旋转酶 …

The ccd locus of the F plasmid codes for two gene products, CcdA and CcdB, which contribute to the plasmid's high stability by post-segregational killing of plasmid-free bacteria. Like the …