2022年4月13日 · Clusters with haematopoietic cells (RUNX1 + CD45 +) and HSCs (cluster 12) are indicated by purple and black circles, respectively. Feature plots identify HSCs by the co-expression of HSC...

2023年8月31日 · Epitope-edited CD45 CAR T cells were fratricide resistant and effective against patient-derived acute myeloid leukemia, B cell lymphoma, and acute T cell leukemia. Epitope-edited hematopoietic stem cells (HSCs) were protected from CAR T cells and, unlike CD45 knockout cells, could engraft, persist, and differentiate in vivo.

2012年6月26日 · Progeny of donor HSC (CD45.2) can be discriminated from recipient cells (CD45.1) by CD45 alleles, and the donor HSCs that were successfully transduced to over-express a test gene are GFP +. The CD45.2 + /GFP + population can then be gated to a PeCy7 versus Pacific Blue dot-plot to analyze distribution of the blood lineages.

2016年5月18日 · Here we use a novel combination of surface markers to isolate individual functional CD45 − and CD45 + pre-HSCs in the E11 mouse aorta–gonad–mesonephros (AGM) region efficiently. By single-cell...

2023年9月20日 · Iomab-B是由Fred Hutchinson癌症研究中心开发的一款靶向CD45的核药，旨在通过同时快速耗竭特异性表达CD45的血液癌、免疫细胞和骨髓干细胞来增加患者接受BMT（Bone marrow transplantation，骨髓移植）的机会，已被美国食品和药物管理局和欧洲药品管理局授予孤 …

The integrin alpha chain subunit protein, CD49f, was identified in 2011 as a marker that could be used to further purify the Lin − CD34 + CD38 − CD45RA − CD90 + HSC population to 1 in 10.5 HSC frequency (Notta et al., 2011). CD49f has been identified as a biomarker of stem cells across systems, including epithelial tissues, cardiac ...

2020年5月28日 · Today, the most prevalent genetic HSC detection method uses congenic C57BL/6 mouse strains that carry different alleles of Ptprc, the gene encoding the pan-leukocyte surface marker CD45 (refs...

CD45 dephosphorylates and downregulates the activity of Src family kinases (SFK) on hematopoietic stem cells (HSCs). This decreases integrin activity and adhesion as occurs, for example, between HSC Integrin alpha 4 beta 1 and reticular cell VCAM-1. CD45 also downregulates osteoclast SFK activity, which allows TRANCE/RANK L to induce the ...

2024年5月26日 · 优化的BE系统能够有效地体外屏蔽HSPC，产生对CD45靶向ADC具有抗性的独特编辑细胞群。 然后，研究人员通过将pyrrolobenzodiazepine二聚体与人源化抗CD45抗体偶联，开发了一种强效的人源化CD45靶向ADC药物CIM053-SG3376。

2022年11月15日 · To protect the hematopoietic system from CD45-directed on-target/off-tumor toxicity, we base-edited the targeted CD45 epitope in human HSCs, achieving the desired Tyr->Cys conversion in 62% of human HSCs (median, 51%-91% range). CD45 edited HSC maintained equivalent colony formation in vitro when compared to CD45 WT HSC.