Use Thermo Scientific™ ProPac™ WCX-10 LC Columns to provide exceptionally high resolution for separations of proteins and their variants. Based on stationary phases composed of 10μm nonporous polymeric beads, ProPac columns resolve isoforms that differ by a …

Cation exchange chromatography is a form of ion exchange chromatography (IEX), which is used to separate molecules based on their net surface charge. Cation exchange chromatography, more specifically, uses a negatively charged ion exchange resin with an affinity for molecules having net positive surface charges.

Cation Exchange Chromatography (CEX) analysis is commonly used to characterize the charge heterogeneity of therapeutic proteins by determining acidic and basic charge variants. Salt gradient and pH gradient are two widely used methods of CEX analysis.

HiTrap SP HP is a strong cation exchange chromatography column for high-resolution, small-scale protein purification. Packed with SP Sepharose High Performance strong cation exchange resin . Small (34 µm) bead size delivers high-performance, high-resolution purifications. Can be used alone or connected in series.

Bio-Rad has a wide selection of cation exchange columns covering a broad range of cation exchange chromatography applications. Column configurations have been optimized for all steps of purification and analysis, from capture through intermediate purification to …

higher proportion of basic residues, cation-exchange chromatography (CEX) is commonly used. We used an Agilent Bio MAb weak cation-exchange (WCX) column to separate charge variants of the monoclonal antibody trastuzumab, marketed as Herceptin, and the subsequent characterization of the variants by reversed-phase liquid

⋙ Antibodix™ WCX and Proteomix® CEX columns can successfully separate mAb variants using an optimized salt gradient and/or pH gradient. ⋙ Monoclonal antibody purity, heterogenity and stability can all be monitored using Sepax's CEX phases.

Cation exchange chromatography is a form of ion exchange chromatography in which a positively charged biomolecule binds to a negatively charged resin. POROS CEX resins are designed for high dynamic binding capacity over a range of pH and conductivity conditions, thereby increasing process development flexibility and manufacturing throughput.

2020年9月14日 · Cation exchange chromatography (CEX) is an essential part of most monoclonal antibody (mAb) purification platforms. Process characterization and root cause investigation of chromatographic unit operations are performed using scale down models (SDM).

Our portfolio of anion exchange (AEX) and cation exchange (CEX) resins, with our multi-modal chromatography resins, offers efficient purification solutions for mAbs, vaccines, plasmid DNA, plasma, and other modalities.