2019年12月2日 · LR73 phagocytes treated with siRNA-mediated reduction in Oxsr1 (b), Stk39 (c) or Wnk1 (d) expression show significantly increased apoptotic cell uptake. The phagocytosis index was normalized to...

2020年3月18日 · LR73 cells (ATCC) were plated at 10 5 per well in 24-well tissue culture plates and cultured for 16 h at 37 °C with 5% CO 2. The cells were then rinsed with PBS, and fresh supernatants taken from...

2018年11月21日 · To identify the pathways potentially involved in efferocytosis, we performed RNA sequencing of LR73 hamster phagocytes engulfing apoptotic human Jurkat cells (to clearly distinguish phagocyte ...

研究者向吞噬细胞LR73中添加了凋亡Jurkat细胞的上清液，并对LR73细胞进行RNA测序(处理4小时后)。 结果发现，相对于对照组，凋亡细胞上清液处理显著改变了吞噬细胞一些基因的表达，功能分析发现这些基因富集到细胞骨架重组、炎症、伤口愈合、组织修复、抗 ...

对33个SLC进行功能分析，发现主要分为两大类：与碳水化合物代谢相关的SLC表达上调，而与氧化磷酸化、脂肪酸氧化相关的SLC表达下调。 验证差异表达基因. 作者重点关注SLC家族的变化，通过体内、体外实验验证RNA-seq的结果。 体外实验：培养小鼠的腹腔巨噬细胞，胞葬凋亡Jurkat细胞后实时定量PCR检测SLC的变化。 体内实验：将凋亡Jurkat注射入小鼠腹腔，2小时后分选CD11b+F4/80+巨噬细胞，实时定量PCR检测分选巨噬细胞SLC的变化。 候选基因功能研究.

为了检测从凋亡细胞中释放出来的保守代谢产物是否会改变附近健康细胞(如吞噬细胞)的基因表达，作者将活的或凋亡的Jurkat T细胞上清液加入吞噬细胞系LR73细胞中（图3a）。

LR73 cells were fixed in 3.7% paraformaldehyde and permeabilized with phosphate-buffered saline/0.1% bovine serum albumin/0.1% Triton X-100. The permeabilized cells were blocked with phosphate-buffered saline/1% bovine serum albumin prior to staining with Alexa Fluor 568-phalloidin (Molecular Probes) and analyzed by using an Olympus confocal ...

2016年11月11日 · To address potential cross-regulation among phagocytes, we initially tested a panel of eleven soluble mediators that have been linked to tissue repair, inflammation dampening, and tissue morphogenesis 13, for their ability to modulate engulfment by LR73 fibroblasts, a non-professional phagocytic cell line.

Here, we present evidence that Rho-GTP levels decrease during engulfment. RhoA seems to negatively affect basal engulfment, such that inhibition of Rho-mediated signaling in phagocytes enhanced the uptake of apoptotic targets. Activation of endogenous Rho or overexpression of constitutively active forms of Rho also inhibited engulfment.

To test whether released apoptotic cell-derived metabolites signal to alter gene expression programs in healthy nearby cells such as phagocytes, we added supernatants from live or apoptotic Jurkat cells (same conditions as untargeted metabolomics) to LR73 cells, a model phagocyte useful in revealing mechanisms/responses after efferocytosis 15 ...