2021年3月31日 · Cyclic GMP-AMP (cGAMP) synthase (cGAS), a cytosolic DNA sensor, catalyzes the synthesis of cyclic dinucleotide cGAMP, which binds and activates Stimulator of interferon (IFN) genes...

2023年1月19日 · Since WTp53 activated the cGAS/STING pathway, we investigated if this resulted in differences in expression of interferon stimulated genes (ISGs) via Nanostring analysis. We observed that WTp53 (in A549 and H1299 cells) had a high correlation with increased expression of ISGs (Figures 1E and 1F).

2018年10月24日 · We further observed a dose-dependent, stress-induced nuclear translocation of cGAS in two human lung cancer cell lines, A549 and PC-9 (Extended Data Fig. 2a–h). DNA binding and enzymatic...

cGAS is required for IFN-γ-induced STING activation. (a) Immunoblots showing cGAS expression in A549 and HCC827 cells treated with or without IFN-γ (100 IU/mL). (b) A549 and HCC827 cells were treated with IFN-γ for 12 h. The cellular localization of cGAS was assessed using fluorescence microscopy (scale bar, 20 μm). DNA was visualized with ...

2024年8月21日 · 羟基脲 (HU) 是一种明确的 cGAS-STING 信号和细胞衰老的诱导剂，用羟基脲 (HU) 处理肠道上皮 DLD1 细胞，激活 STING 信号并导致 TBK1 和 IRF3 的磷酸化 (图 1A)。 IRF3 在长时间观测期间保持持续活跃 (图 1A)。 IRF3 基因敲除显著抑制 DLD1 细胞中 DNA 损伤诱导的衰老，而重新引入 IRF3 可以逆转这一效应 (图 1B 和 C)。 上述结果表明，cGAS-STING-IRF3 信号在诱导 DNA 损伤后的细胞衰老中起着显著作用。 为进一步表征 IRF3 在 HU 诱导的衰老中 …

2017年6月15日 · The A549 cell line expresses functional cGAS that is capable of producing cGAMP in the presence of poly(dA:dT), ATP, and GTP when compared to THP-1 cells, a known cGAS-expressing human...

Cyclic guanosine monophosphate-adenosine monophosphate synthase (cGAS) is a newly identified cytosolic DNA sensor, but its function in lung epithelial cells is relatively unknown. In the present study, the effects of lipopolysaccharide (LPS) on the expression and function of cGAS in the A549 lung ep …

In the present study, cGAS mediates A549 cellular responses against cytosolic DNA, providing evidence that cGAS are active in A549 cells. Although DNA is absent in the cytoplasm of lung epithelial cells under normal conditions, it may be released into the cytoplasm to cause immune responses under certain abnormal conditions, such as infection ...

2020年6月27日 · 更加令人振奋的是，进一步应用人源化小鼠模型发现STING激动剂可以显著提高CAR-T细胞治疗A549实体瘤的效率。 该研究从免疫治疗面临的临床问题出发，深入研究了CD8+ T细胞内源的cGAS-STING信号通路对T细胞的作用及机制；揭示了cGAS-STING通路通过维 …

在本研究中，研究了脂多糖（lps）对a549肺上皮细胞系中cgas表达和功能的影响。 用不同浓度（例如100、200、400和800 ng / ml）的LPS处理细胞，并通过蛋白质印迹分析和逆转录定量聚合酶链反应（RT-qPCR）检查cGAS表达水平。