cgas-sting通路是细胞内重要的免疫信号通路，负责识别病毒和细菌的双链dna（dsdna）。 cGAS结合dsDNA后生成环状GMP-AMP（cGAMP），激活STING（刺激性基因）。 活化的STING进一步招募TBK1并激活IRF3，促进I型干扰素和…

2024年2月28日 · Cyclic GMP-AMP synthase (cGAS) senses aberrant DNA during infection, cancer and inflammatory disease, and initiates potent innate immune responses through the...

2019年10月29日 · We find that cancer cells produce cGAMP that is transferred via gap junctions to tumor-associated dendritic cells (DCs) and macrophages, which respond by producing type I IFN in situ. Cancer-cell-intrinsic expression of cGAS, but not STING, promotes infiltration by effector CD8 + T cells and consequently results in prolonged survival.

2024年3月12日 · The cGAS-STING immunostimulation pathway plays a critical role in innate immunity that mediates anti-cancer therapies, including ICB. Upon dsDNA binding, cGAS is activated to synthesize cGAMP, which then stimulates STING to trigger antitumor IFN-I …

2024年12月27日 · 对cGAS-STING通路的结构和分子生物学的深入理解，使得开发选择性小分子抑制剂成为可能，这些抑制剂可以在多种人类炎症性疾病中靶向cGAS-STING轴。 在本文中，我们将概述cGAS-STING信号级联的主要元素，并探讨cGAS-STING活性与各种自身炎症、自身免疫性和退行性疾病相关的一般机制。 最后，我们将概述最近开发的cGAS和STING拮抗剂的化学特性，并总结它们的潜在临床应用。 1 介绍. 外源性DNA的检测是众多生物体免疫反应的关键环节。 在 …

2024年8月2日 · Hyperactivation of cGAS resulted in the formation of tertiary lymphoid structures (TLSs) in the lung, which enhanced antibody responses and antitumor immunity. CCL5-producing endothelial cells in the lung promoted CD8 + T cell recruitment to TLSs via CCR5 signaling, which was induced by T cell–intrinsic cGAS expression.

2020年8月24日 · Antitumor immunity depends on the activation of the cGAS-STING pathway, as STING-deficient mice fail to stimulate tumor-infiltrating dendritic cells (DCs) to activate CD8 + T cells. STING...

Here, we use micronuclei purification to show that the endoplasmic reticulum (ER)-associated nuclease TREX1 inhibits cGAS activation at micronuclei by degrading micronuclear DNA upon micronuclear envelope rupture. We demonstrate that the ER accesses ruptured micronuclei and plays a critical role in enabling TREX1 nucleolytic attack.

2024年4月15日 · As a key sensor of double-stranded DNA (dsDNA), cyclic GMP-AMP synthase (cGAS) detects cytosolic dsDNA and initiates the synthesis of 2'3' cyclic GMP-AMP (cGAMP) that activates the stimulator of interferon genes (STING). This finally promotes the production of type I interferons (IFN-I) that is cruc …

流式细胞仪 的工作原理是将待测细胞经特异性 荧光染料 染色后放入样品管中，在气体的压力下进入充满鞘液的流动室。 在鞘液的约束下细胞排成单列由流动室的喷嘴喷出，形成细胞柱，后者与入射的激光束垂直相交，液柱中的细胞被激光激发产生荧光。 仪器中一系列光学系统 (透镜、光阑、滤片和检测器等)收集荧光、光散射、光吸收或细胞电阻抗等信号，计算机系统进行收集、储存、显示并分析被测定的各种信号，对各种指标做出统计分析。 释义. 科研型 流式细胞仪 还可以根 …