环鸟苷酸-腺苷酸合成酶（Cyclic GMP-AMP synthase，cGAS）能够识别细胞质内的双链DNA，包括外源的双链DNA（如病毒、细菌、寄生虫等）和自身的双链DNA（如受损的线粒体DNA、微核DNA【2】、反转录转座子等）。 cGAS和双链的DNA结合后会催化第二信使环鸟苷酸-腺苷酸的产生，产生的环鸟苷酸-腺苷酸会结合和激活干扰素刺激蛋白 STING，STING蛋白会招募丝氨酸苏氨酸蛋白激酶（TBK1），从而来激活 IRF3，最终导致I型干扰素和免疫因子的产生。 近年来， …

2024年2月28日 · Here we show that the ubiquitin proteasomal system (UPS) degrades nuclear cGAS in cycling cells. We identify SPSB3 as the cGAS-targeting substrate receptor that associates with the cullin–RING...

Pathogenic and other cytoplasmic DNAs activate the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway to induce inflammation via transcriptional activation by IRF3 and nuclear factor κB (NF-κB), but the functional consequences of exposing cGAS to chromosomes upon mitotic nuclear envelope breakdown are unknown.

2023年3月2日 · Here we show that cGAS is localized to mitochondria and protects hepatocellular carcinoma cells from ferroptosis in vitro and in vivo. cGAS anchors to the outer mitochondrial membrane where it...

2017年7月24日 · Here, we report that cGAS localizes to micronuclei arising from genome instability in a mouse model of monogenic autoinflammation, after exogenous DNA damage and spontaneously in human cancer...

Expresses catalytically-active GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

cGAS（cyclic GMP–AMP synthase）作为胞质内环状GMP-AMP合成酶被发现，其可识别结合胞质内DNA发生活化，将胞质内AMP与GMP环化产生2’,3’-cGAMP。 cGAMP 作为第二信号继续激活STING（stimulator of interferon genes），继而通过 IRF3 和 TBK1 促进干扰素 IFN 表达发挥抗病毒作用。 在常规情况下，细胞胞质内仅存在微量的DNA，故以cGAS为主的胞质DNA感受器处于未活化状态。 当细胞处于某些应激状态，如DNA病毒感染，使胞质内DNA含量上升，即可通 …

2018年10月25日 · 研究者进一步筛选了89个酪氨酸激酶shRNA对GFP-cGAS入核的调控作用，发现下调BLK（B-lymphoid tyrosine kinase）表达可显著增强cGAS入核，同时cGAS Y215位点的磷酸化水平降低。

CGAS (cyclic GMP-AMP synthase) has been identified as a sensor of double-stranded DNA (dsDNA) located in the cytoplasm or micronuclei to induce innate immunity and cellular senescence [12, 13, 24, 25]. Upon DNA binding, CGAS is activated to generate cGAMP from guanosine triphosphate (GTP) and adenosine triphosphate (ATP) [24–26].

2021年4月8日 · Here, we have discovered a spherical ER membranous biocondensate with puzzle-like structures caused by condensation of the ER-resident stimulator of interferon genes (STING) in DNA virus-infected...