An immunoreceptor tyrosine-based activation motif (ITAM) is a conserved sequence of four amino acids that is repeated twice in the cytoplasmic tails of non-catalytic tyrosine-phosphorylated receptors, cell-surface proteins found mainly on immune cells. [1] .

Signal transduction by the T-cell antigen receptor (TCR) is initiated by phosphorylation of conserved motifs (ITAMs) contained within the cytoplasmic domains of the invariant subunits. TCR complexes contain a total of 10 ITAMs and this unusual ...

In the immune system and beyond, signals transduced through cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs) control a variety of key cellular responses, including phagocytosis and degranulation, cell migration and adhesion, proliferation and differentiation, and gene induction [1].

2023年11月9日 · Unexpectedly, we found that T cells expressing TCRs containing inactivated (non-signaling) CD3ζ ITAMs (6F-CD3ζ) exhibited reduced ability to discriminate between low- and high-affinity ligands,...

2007年2月1日 · In the immune system and beyond, signals transduced through cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs) control a variety of key cellular responses, including phagocytosis and degranulation, cell migration and adhesion, proliferation and differentiation, and gene induction [1].

There is ample evidence that ITAMs are a critical component of receptor complexes involved in linking the ligand-binding portion of the receptor to the activation of intracellular signaling cascades, including those that contribute to cellular activation and cell survival.

2023年11月9日 · 美国国家儿童健康和人类发展研究所Paul E. Love课题组发现，CD3ζ 免疫受体酪氨酸激活基序 (ITAM)通过抑制低亲和肽的T细胞抗原受体 (TCR)信号传导，来实现配体的识别和拮抗。 相关论文于2023年11月9日发表于国际顶尖学术期刊《自然—免疫学》杂志上。 他们使用条件“开关”小鼠模型评估了灭活三个CD3ζ链ITAM对TCR信号传导和T细胞效应反应的影响。 出乎意料的是，他们发现T细胞表达含有失活 (非信号)CD3ζ ITAMs (6F-CD3ζ)的TCR，表现出区分低亲和 …

为了对 TCR-CD3复合物 上所有磷酸化位点同时进行定量检测，研究团队发展了一种targeted-IP-multiplex-light-absolute-quantitative mass spectrometry (TIMLAQ-MS)质谱方法，在一次检测中可以对来自不同时间点刺激样品中所有CD3 ITAMs的磷酸化位点进行绝对定量。 在TIMLAQ-MS中，6种不同浓度的合成的磷酸化和非磷酸化的CD3肽段作为标准品和来自四个不同时间点刺激的T细胞的TCR-CD3样品同时进行质谱样本的处理标记和检测。 利用标准品做出每一个磷酸化位点 …

T 细胞抗原受体 （TCR） 包含分布在 6 个 CD3 亚基内的 10 个基于免疫受体酪氨酸的激活基序 （ITAM） 信号序列;然而，这种结构复杂性和多样性的原因尚不清楚。 在这里，我们使用条件“开关”小鼠模型评估了灭活三个 CD3ζ 链 ITAM 对 TCR 信号传导和 T 细胞效应器反应的影响。 出乎意料的是，我们发现表达含有灭活（非信号传导）CD3ζ ITAM （6F-CD3ζ） 的 TCR 的 T 细胞表现出区分低亲和力和高亲和力配体的能力降低，导致信号转导和细胞因子对低亲和力配体的反应增 …

The ζ chain is distinct in having only a short extracellular domain, but it has three ITAMs in its cytoplasmic domain. The CD3 chains have negatively charged acidic residues in their transmembrane domains, which are able to interact with the positive charges of the α and β chains, as shown in Fig. 6.8 .