2022年5月4日 · The results showed that displacement is the largest and fastest when DsrA (H-DA) is challenged by ChiX (CX), in agreement with the sRNA concentration dependence (Fig. 1b). By contrast, when...

2009年9月1日 · Abundance of the ChiX sRNA is regulated by a target decoy, an inducible OFF switch. What is the basis for this chitobiose-induced relief of chiP repression? The answer lies in the characteristics of degradation of this sRNA, and uncovers a unique OFF switch for an sRNA whose default expression appears to be ON.

2016年9月19日 · A chbB–chbC intercistronic spacer sequence in the polycistronic chb mRNA is recognized as a target by ChiX and elicits the degradation of the sRNA upon pairing with it, thereby depleting the ...

2024年9月19日 · Two base-pairing targets of the ChiX sRNA are the 5′ UTR of the chiZPQ mRNA, which encodes an outer membrane chitoporin (ChiP) and a lipoprotein (ChiQ), and the chbBC intergenic region of the chbBCARFG mRNA, which encodes the ChbBCA proteins required for chitosugar transport across the inner membrane as well as …

Small RNAs (sRNAs) associated with the RNA chaperon protein Hfq are key posttranscriptional regulators of gene expression in bacteria. Deciphering the sRNA-target interactome is an essential step toward understanding the roles of sRNAs in the cellular networks.

2015年10月10日 · ChiX sRNA is turned off by an RNA decoy, made under specific growth conditions, that interacts with the sRNA, destabilizing the sRNA terminator and leading to degradation of the sRNA. In growing cells, ChiX levels are high enough to fully repress its target(s); repression is only relieved in response to signals that induce the decoy, making ...

2012年8月15日 · By studying the mechanism by which ChiX, upon pairing near the 5' end of the transcript, represses the distal gene in the operon, we discovered that the action of the sRNA induces Rho-dependent transcription termination within the chiP cistron.

In chiP reporter cells, endogenous ChiX sRNA is constitutively expressed and strongly inhibits chiP expression, resulting in nonfluorescent cells. Deletion of chiX led to highly fluorescent cells for both mCherry and GFP (Fig. 1B), consistent with the repressive roles of ChiX in inhibiting translation and promoting decay of chiP mRNA .

2024年9月19日 · Two base-pairing targets of the ChiX sRNA are the 5′ UTR of the chiZPQ mRNA, which encodes an outer membrane chitoporin (ChiP) and a lipoprotein (ChiQ), and the chbBC intergenic region of the chbBCARFG mRNA, which encodes the ChbBCA proteins required for chitosugar transport across the inner membrane as well as …

2022年5月4日 · The results showed that displacement is the largest and fastest when DsrA (H-DA) is challenged by ChiX (CX), in agreement with the sRNA concentration dependence (Fig. 1b). By contrast, when ChiX was challenged by DsrA (H-CX vs DA), very little ChiX was removed, comparable to control experiments in the absence of a competitor (H-DA and H-CX).