2023年10月24日 · Overexpression of PSMD2 significantly facilitated the degradation of endogenous MYH9 in HONE1 cells treated with CHX (Fig. 5E). Fig. 5: DNAJA4 recruits …

2024年2月6日 · Mass spectrometry and Co-IP assays reveal that USP10 deubiquitinates and stabilizes ATMIN protein, resulting the high-ATMIN expression in NPC. Knockdown of ATMIN suppresses the cell proliferation...

2022年1月25日 · Overexpression of USP44 significantly promoted the degradation of Ku80, and knockout of USP44 inhibited the degradation of endogenous and exogenous Ku80 through treatment with CHX, which means ...

NPM1 induced the ubiquitin-mediated proteasome degradation of p53 by recruiting Mdm2. A, C. Cycloheximide (CHX) chase analysis of the half-life of p53 in control and NPM1-overexpressing 5-8F and HONE1 cells. CHX: 50 μg/ml. B, D. The half-life of p53 in 5-8F and HONE1 cells. E, F.

2024年7月8日 · To investigate whether the G3BP1 K76R mutant would affect the stability of G3BP1, we conducted western blotting assay in HONE1 and SUNE1 cells that transfected with siRNA targeting G3BP1 (Figure S7A, Supporting Information) and then overexpressed with G3BP1- WT or K76R mutant following CHX treatment.

sumo2的过表达在用放线菌酮（chx）处理的hne2和cne2细胞中诱导glut1降解。 泛素化实验表明，SUMO2的过表达加速了GLUT1的泛素化（图7E）。 敲低circRNF13减弱了用sicircRNF13和SUMO2过表达载体共转染的HNE2和CNE2细胞中SUMO2诱导的GLUT1泛素化水平（图7F）。

2022年2月28日 · We then treated NPC cells with cycloheximide (CHX) to identify the effect of circCAMSAP1 and SERPINH1 on the stability of the c-Myc protein in NPC cells. The results showed that circCAMSAP1 and SERPINH1 could inhibit …

2024年9月1日 · In this study, we first established GEM-resistant (GEM-R) NPC cell lines (HONE1/GEM-R and SUNE1/GEM-R) and conducted lncRNA profiles using Illumina sequencing. Among 221 dysregulated lncRNAs, DYNLRB2-AS1 was identified as a significantly upregulated lncRNA in NPC/GEM-R cells compared to parental cells.

2022年11月21日 · GSDME-dependent pyroptosis is a critical determinant of radiosensitivity in NPC, and is modulated by OTUD4 via deubiquitinating and stabilizing GSDME. These findings reveal a promising novel direction to investigate radioresistance and suggest potential therapeutic targets for sensitizing NPC to radiotherapy.

2022年4月28日 · The ectopic expression of p53 mediated by plasmid transfection in CNE2 and HONE1 cells greatly suppressed PFKFB3 expression at the mRNA and protein levels compared to GADD45A expression, a target gene of p53 (Fig. 3 C and D).