2017年12月22日 · We report a new online capillary isoelectric focusing-mass spectrometry (CIEF-MS) method for monoclonal antibody (mAb) charge variant analysis using an electrokinetically pumped sheath-flow nanospray ion source and a time-of-flight MS with pressure-assisted chemical mobilization.

Experience icIEF separation and UV detection coupled with mass spectrometry identification on the ZenoTOF 7600 system. One workflow, on a single platform, enables rapid monitoring of intact biotherapeutics and identification of charge variants throughout the drug development pipeline.

Capillary isoelectric focusing (CIEF) provides automation, repeatable detection and quantification of recombinant proteins, easy documentation and archiving of analysis

2018年2月6日 · We report a new online capillary isoelectric focusing-mass spectrometry (CIEF-MS) method for monoclonal antibody (mAb) charge variant analysis using an electrokinetically pumped sheath-flow nanospray ion source and a time-of-flight MS with pressure-assisted chemical mobilization.

In this mini review, we summarize the technical improvements of cIEF-ESI-MS for characterizing proteoforms and highlight some recent applications to hydrophobic proteins, urinary albumin variants, charge variants of monoclonal antibodies, and large-scale TDP of complex proteomes.

2025年1月1日 · Capillary isoelectric focusing (cIEF) is a high-resolution separation for proteins and peptides which is capable of separating proteoforms. MS detection coupled to cIEF can separate, detect, and characterize proteoforms at the molecular level.

Capillary Isoelectric Focusing (cIEF) is a technique with high peak capacity and high-resolution power based on the separation of amphoteric molecules according to their pI, which can effectively distinguish between molecules that have minor differences in the net charge.

2018年8月7日 · Capillary isoelectric focusing directly coupled to high resolution mass spectrometry (cIEF-MS) provides information on amphoteric molecules, including isoelectric point and accurate mass, which enables structural interrogation of biopolymer pI variants. The coupling of cIEF with MS was facilitated b …

2022年6月4日 · Here, we report a novel online capillary isoelectric focusing-mass spectrometry (CIEF-MS) method for mAb charge variant analysis using an electrokinetically pumped sheath-flow nanospray ion source on a time-of-flight (TOF) MS with a …

Different separation modes exist in CE: capillary zone electrophoresis (CZE) with separation on the basis of the charge/mass ratio, capillary isoelectric focusing (CIEF) based on the isoelectric point, capillary isotachophoresis based on analyte conductivity, and micellar electrokinetic capillary chromatography (MECC) based on hydrophobicity.