Capillary isoelectric focusing (CIEF) provides automation, repeatable detection and quantification of recombinant proteins, easy documentation and archiving of analysis

Here, we introduce the application of capillary isoelectric focusing (cIEF) for antibody charge heterogeneity analysis. It is widely used for the characterization of therapeutic mAbs. By determining the pI of mAb charge isoforms, cIEF provides information crucial for establishing identity, antibody impurity , post-translational modifications ...

This application note demonstrates the suitability of capillary isoelectric focusing (cIEF) to assess the charge heterogeneity of different types of monoclonal antibodies. Here, we analyzed the charge variants of 3 different IgGs, including an innovator

Detection of Impurities and Contaminants: cIEF serves as a powerful tool for detecting impurities and contaminants in biopharmaceutical products. It can identify trace amounts of host cell proteins, residual DNA, aggregates, or other impurities that …

icIEF具有方法开发简单、通量高、等电点 (pI)测定和定量重现性好、易于开发平台方法等优点。 去除了传统clEF迁移步骤，将分析时间从40到60分钟减少到每个样品5到15分钟。 icIEF方法每次进样分析时间短，并能够实时观察整个聚焦过程，很容易地识别出峰型不重现的原因，如蛋白质聚集情况下，可以通过优化聚焦时间或添加稳定剂来避免。 icIEF技术成功商业化后，已经逐渐取代传统的cIEF技术，该方法由于性能优异还收录于2020版最新药典，在生物制药行业得到了广泛应 …

Capillary isoelectric focusing (cIEF) is a technique that allows quantitative, experimental analysis of the isoelectricfocusing point (pI) and charge variants of a protein. Many of the reagents and supplies required to prepare samples for cIEF analysis are available from SCIEX.

2011年11月1日 · iCIEF separation is conducted by focusing isoforms in an ampholytic pH gradient through an applied electric field. Following the focusing step is a detection step, which in conventional CIEF would entail a chemical or pressure mobilization across a fixed UV detector 4,.

To support analysis of the charge heterogeneity of USP mAbs, we developed methods for cIEF using the SCIEX platform (PA 800 Plus) and icIEF using Bio-Techne’s Protein Simple Maurice and iCE3 platforms (Table 3).

2024年11月15日 · Capillary isoelectric focusing (cIEF) and the more recently developed icIEF are emerging applications to characterize the charge heterogeneity of mAbs 8, 9, 10 and their...

2022年5月1日 · Capillary isoelectric focusing with whole-column imaging detection (icIEF) technology is now the mainstream technology to perform analytical isoelectric focusing analysis in pharmaceutical industry.