Muscleblind (mbl) is an essential muscle and neuronal splicing regulator. Mbl hosts multiple circular RNAs (circRNAs), including circMbl, which is conserved from flies to humans. Here, we show that mbl- derived circRNAs are key regulators of MBL by cis - and trans- …

2014年10月2日 · Modulation of MBL levels strongly affects circMbl biosynthesis, and this effect is dependent on the MBL binding sites. Together, our data suggest that circRNAs can function in gene regulation by competing with linear splicing. Furthermore, we identified muscleblind as a factor involved in circRNA biogenesis. Copyright © 2014 Elsevier Inc.

2022年4月26日 · Muscleblind (mbl) is an essential muscle and neuronal splicing regulator. Mbl hosts multiple circular RNAs (circRNAs), including circMbl, which is conserved from flies to humans. Here, we show that mbl-derived circRNAs are key regulators of MBL by cis- and trans-acting mechanisms. By generating fly …

2019年8月8日 · One of the first examples of a circRNA functioning as a protein sponge came from a study of the splicing factor protein gene that encodes muscleblind (mbl) in D. melanogaster; mbl is a...

第一个作为蛋白海绵被发现的circular RNA是circMbl，它与MBL (multifunctional protein muscleblind) 之间存在反馈环调控。 MBL通过结合在circMbl 内含子的侧端，促进circMbl的生物合成，相应地，增加MBL会减少Mbl mRNA 剪接，而促进circMbl反向剪接，从而提高circMbl表达；反之，高表达的circMbl结合MBL，隔离该蛋白，从而影响其在别的神经元发挥功能。

2014年10月2日 · Modulation of MBL levels strongly affects circMbl biosynthesis, and this effect is dependent on the MBL binding sites. Together, our data suggest that circRNAs can function in gene regulation by competing with linear splicing. Furthermore, we identified muscleblind as a factor involved in circRNA biogenesis.

如在黑腹果蝇中，RBP Muscleblind （Mbl）通过与侧翼内含子中的Mbl结合位点结合，促进了circRNA circMbl的产生。 与单个基因的观察不同，QKI与人类细胞上皮-间质转化过程中数百个circRNA的上调有关。

2021年3月5日 · Circular RNAs (circRNAs), a novel type of endogenous RNAs with covalently closed-loop structures, have become a new research hotspot in the RNA world. Their diversity, stability, evolutionary conservation, and cell type- or tissue-specific expression patterns endow circRNAs with various important biological functions.

Muscleblind (mbl) is an essential muscle and neuronal splicing regulator. Mbl hosts multiple circular RNAs (circRNAs), including circMbl, which is conserved from flies to humans. Here, we show that mbl -derived circRNAs are key regulators of MBL by cis - and trans …

该circRNA（circMbl）及其侧翼内含子含有保守的肌肉盲结合位点，这些结合位点被MBL强烈而特异性地结合。 MBL水平的调节强烈影响circMbl的生物合成，这种作用取决于MBL结合位点。