ClpXP, an ATP-powered unfolding and protein-degradation machine
ClpXP is a AAA+ protease that uses the energy of ATP binding and hydrolysis to perform mechanical work during targeted protein degradation within cells. ClpXP consists of hexamers …
ClpXP, an ATP-powered unfolding and protein-degradation …
2012年1月1日 · ClpXP is a AAA+ protease that uses the energy of ATP binding and hydrolysis to perform mechanical work during targeted protein degradation within cells. ClpXP consists of …
Structure and function of ClpXP, a AAA+ proteolytic machine …
ClpXP is an archetypical and well-studied AAA+ protease present in most eubacteria and the mitochondria of eukaryotic cells (Baker and Sauer, 2012; Olivares et al., 2018). It consists of …
Cryo-EM structure of the ClpXP protein degradation machinery
2019年10月3日 · The ClpXP machinery is a two-component protease complex that performs targeted protein degradation in bacteria and mitochondria. The complex consists of the AAA+ …
Mitochondrial ClpP serine protease-biological function and
2020年10月9日 · Mitochondrial ClpP is a stable heptamer ring with peptidase activity that forms a multimeric complex with the ATP-dependent unfoldase ClpX (ClpXP) leading to proteolytic …
Cell Chem Bio︱中国科学院上海药物研究所杨财广课题组发现线粒 …
ClpP 是高度保守的 丝氨酸蛋白水解酶，广泛存在原核生物和真核生物中。 十四聚体的ClpP与六聚体的分子伴侣ClpX形成ClpXP复合物，负责水解错误折叠或异常的蛋白。 杨财广组早期针对 …
Structure and function of ClpXP, a AAA+ proteolytic machine
2021年12月21日 · ClpXP is an archetypical AAA+ protease, consisting of ClpX and ClpP. ClpX is an ATP-dependent protein unfoldase and polypeptide translocase, whereas ClpP is a self …
Optimization of ClpXP activity and protein synthesis in an
2018年2月22日 · We improved the activity of ClpXP in cell extract by adding exogenous ATP and an energy regeneration system. We then established conditions for both protein synthesis, and …
ClpX(P) Generates Mechanical Force to Unfold and Translocate Its ...
2011年4月29日 · We report direct observations of mechanical, force-induced protein unfolding by the ClpX unfoldase from E. coli, alone, and in complex with the ClpP peptidase. ClpX …
Proteomic Discovery of Cellular Substrates of the ClpXP …
2003年3月1日 · Here, we report the identification of more than 50 E. coli proteins that are trapped in a ClpX-dependent fashion within an active-site mutant of ClpP. Analysis of these ClpXP …