Our results indicate that ectodomain shedding is a highly regulated process that can be stimulated by EGFR signalling pathways and integrin ligation. A wide repertoire of transmembrane proteins are proteolytically released from the cell surface by a process known as 'ectodomain shedding', under both normal and pathophysiological conditions.

2021年4月7日 · The proteolytic release of the soluble cMET (sMET) ectodomain by metalloproteases, a process called ectodomain shedding, reflects the malignant potential of tumour cells. sMET can be detected in the human circulation and has been proposed as biomarker in several cancers.

2006年7月15日 · We hypothesized that c-Met overexpression in cancer might result in increased ectodomain shedding, and that its measure could be a useful biomarker of tumor progression. Experimental design: We developed a sensitive electrochemiluminescent immunoassay to quantitate c-Met protein in cell lysates, culture supernatants, and biological samples.

2006年7月20日 · c-Met shedding correlates with tumor burden in tumor xenograft mouse models. We observed that cultured tumor cells expressing c-Met tended to shed more c-Met ectodomain than their normal tissue counterparts, independent of changes in overall c-Met expression level, and that this tendency was enhanced with increasingly malignant phenotype.

Shedding of soluble c-Met associates with total c-Met amount in pre-clinical models, and soluble c-Met correlates with both c-Met expression level and tumor size in human, high soluble c-Met predicts poorer outcome.

2009年12月10日 · A more general role of shedding processes in context with infection by H. pylori is supported by the finding that shedding of c-Met and E-cadherin was neither dependent on CagA nor on the presence of a functional T4SS. Thus, it is conceivable that enhanced shedding is provoked by any H. pylori strain independent of their virulence. To address ...

2001年3月15日 · We show that epidermal growth factor (EGF) receptor activation, either directly by EGF or indirectly via the G-protein coupled receptor (GPCR) agonist lysophosphatidic acid (LPA), induces cleavage of Met through activation of the Erk MAP kinase signalling cascade.

1995年9月28日 · By Western blotting of the conditioned media and cell lysates of several cell lines expressing the hepatocyte growth factor receptor, we found that suramin, a pharmacological agent that inhibits the activity of many growth factors, was able to …

Soluble Met (s-Met) is generated via c-Met ectodomain shedding. c-Met is initially synthesized as a single-chain intracellular precursor and subsequently undergoes proteolytic processing at different stages during intracellular trafficking , leading to the presentation of …

2021年6月1日 · In the model of partial hepatectomy, double deficient mice exhibited decreased AKT phosphorylation, decreased release of EGFR activating factors and lower shedding of HGF receptor c-Met. Thus,...