T-Coffee is a multiple sequence alignment server. It can align Protein, DNA and RNA sequences. You can use T-Coffee to align sequences or to combine the output of your favorite alignment methods into one unique alignment.

M-Coffee Aligns DNA, RNA or Proteins by combining the output of popular aligners

Sequences input Paste or upload your set of sequences in FASTA format Sequences to align Click here to use the sample file

1. Choose your T-Coffee flavor. T-Coffee can align protein sequences as well as DNA/RNA sequences, also different modes are available. In the main page choose the T-Coffee mode according your requirements.

T-Coffee Aligns DNA, RNA or Proteins using the default T-Coffee. Sequences input Paste or upload your set of sequences in FASTA format Sequences to align Click here to use the sample file

T-Coffee Server. Home; History; Tutorial; References; Contacts; Projects; Download; TCS Evaluates your Alignment and outputs a Colored version indicating the local reliability. Alignment input Paste or upload your Multiple Sequence Alignment in CLUSTAL, FASTA, MSF, NEXUS or PHYLIP format.

iRMSD-APDB Evaluates Multiple Sequence Alignment using structural information with APDB and iRMSD.

What is T-Coffee? T-Coffee is a multiple sequence alignment package. You can use T-Coffee to align sequences or to combine the output of your favorite alignment methods (Clustal, Mafft, Probcons, Muscle, etc.) into one unique alignment (M-coffee).

The web server allows the structure-based alignment of multiple RNAs combining the SARA and the T-Coffee framework . SARA executes a structural superposition of ribose C3' carbons to obtain the equivalences needed to return a structure-based alignment.

The T-RMSD web server allows a fine-grained structural classification of proteins using the T-RMSD method (Tree based on Root Mean Square Deviation), see . Providing a set of proteins sequences, it produces a structural tree with support values for each cluster node, reminiscent to bootstrap values.