2014年1月1日 · While coilin KD enhanced TRV, TBRV, TGMV, and BSMV pathogenicity, it had the completely opposite effect on PVY and TVCV, causing attenuation of infection (or significant delay in its development).

We have shown by Western blot utilizing siRNA KD and coilin KO cell lines, Ni-NTA pulldown assays, and in vitro SUMOylation experiments that coilin plays a role in protein SUMOylation. The expression of DGCR8 is tightly regulated because it is required for normal miRNA biogenesis and normal cell functions.

Coilin-silenced (knocked down, KD) transgenic plants were generated by transgenic expression of two independent hairpin RNA constructs, which were designed to avoid “off-target” silencing.

2011年10月3日 · Coilin is considered to be the CB marker protein and is essential for proper CB formation and composition in mammalian cells. In order to characterize the role of coilin phosphorylation on CB formation, we evaluated various …

In this brief review, I focus on coilin and Cajal bodies. Coilin is a key scaffolding protein of Cajal bodies that is evolutionarily conserved in metazoans. Cajal bodies are thought to be one of the archetypal nuclear structures involved in the metabolism of several short non …

2015年6月12日 · Coilin is able to interact with mdm2/hdm2 ubiquitin ligase that ubiquitinates coilin resulting in its proteasomal degradation as demonstrated by its sensitivity to inhibitors, such as MG132 and...

2014年11月6日 · Robust detection of snRNA transcripts correlated with coilin ChIP-seq peaks on snRNA genes, indicating that coilin binding to nascent snRNAs is a site-specific CB nucleator. Surprisingly, several hundred small nucleolar RNAs (snoRNAs) were identified as coilin interactors, including numerous unannotated mouse and human snoRNAs.

We show that the CB protein coilin interacts with poly (ADP-ribose) polymerase 1 (PARP1), redistributes it to the nucleolus and modifies its function, and that these events are accompanied by substantial increases in endogenous concentrations of salicylic acid (SA), activation of SA-responsive gene expression and callose deposition leading to th...

2022年7月7日 · Here we report that coilin is a regulator of miR-517-3p, sFlt-1, TNIP1, TNFSF15 and NF-κB activation, and this regulation is influenced by hypoxia. We also report that coilin and CBs are induced in the reduced uterine perfusion pressure (RUPP) rat model of PE.

Coilin-silenced (knocked down, KD) transgenic plants were generated by transgenic expression of two independent hairpin RNA constructs, which were designed to avoid “off-target” silencing.