
What is the difference between sequence, reads, and contigs of …
2015年5月19日 · Can someone explain the differences between sequence, reads, and contigs of genetic material such as DNA, if possible with an example? I am new to bioinformatics, and I …
dna sequencing - What is the difference between contig- and read …
2017年6月1日 · I have never heard the term “contig based alignment”, and your question is the only Google hit of this exact query (apart from a 2012 patent application). That said, and …
dna sequencing - What is the difference between sequence …
2015年11月9日 · Fig1: Screen capture from DNA Baser Assembler of an 'assembly to reference'. The reference is in purple color. The forward and reverse sequences are red and green. The …
An example for N50? Why do we need it? - Biology Stack Exchange
2015年5月9日 · Contig or scaffold N50 is a weighted median statistic such that 50% of the entire assembly is contained in contigs or scaffolds equal to or larger than this value. Mathematically: …
homework - Understanding Contig NG50 - Biology Stack Exchange
2021年7月3日 · Contig NG50 is defined as the length at which half of the predicted genome size is contained in contigs longer than this length. I can’t understand this and googling returned …
bioinformatics - What is "contigs" in Picard's ReorderSAM?
In whole-genome assembly, the BAC fragments (red line segments) and the reads from five individuals (black line segments) are combined to produce a contig and a consensus …
What's the difference between shotgun sequencing and clone …
In short: the basis of the process is the same in both, the difference relies that in the clone based sequencing you make DNA library of the pieces of DNA clones you got from the sequence you …
How to identify an unknown species from its genome sequence
2020年10月6日 · I have tried using ncbi's BLASTn on one of my contigs with default parameters, on nucleotide collection database. The contig length is about 360 000 bases long. I let the …
What exactly are computers used for in DNA sequencing?
As a consequence, you instead end up with distinct, contiguous blocks (“contigs”) of mapped reads. Each contig is a sequence fragment, like reads, but much larger (and hopefully with …
How exactly are gaps defined in genomics? - Biology Stack Exchange
2016年4月10日 · The word ‘gap’ can be used both loosely and in specific genomic contexts, as listed by @Student T. The usage that seems to relate to your question — Sequence Coverage …