A novel cereblon modulator recruits GSPT1 to the CRL4 CRBN …
2016年6月22日 · To identify potential substrates of CRL4 CRBN regulated by CC-885, we generated 293T HEK cells stably expressing Flag- and haemagglutinin (HA)-double-tagged cereblon (FH–CRBN).
Lenalidomide Causes Selective Degradation of IKZF1 and IKZF3 in ...
2013年11月28日 · IKZF3 was ubiquitinated in vitro when coimmunoprecipitated with CRBN from 293T cells, demonstrating that IKZF3 is an enzymatic substrate of the CRBN complex . These studies demonstrate that lenalidomide causes increased binding of IKZF1 and IKZF3 to CRBN and promotes their ubiquitination and degradation.
Cereblon attenuates DNA damage-induced apoptosis by …
2019年1月25日 · Human embryonic kidney (HEK) 293T cells were co-transfected with green fluorescent protein (GFP) or GFP-CRBN and Flag-p53 for 48 h and the resulting cell lysates were subjected to...
Discovery of novel potential CRBN modulators through structure …
2022年12月1日 · CRBN protein is an E3 ubiquitin ligase which plays an important role in the ubiquitin-proteasome system of eukaryotic cells. Small molecules can modulate CRBN and induce multiple target proteins to bind with CRBN, which contributes to ubiquitination and …
Genome-wide screening reveals a role for subcellular ... - Nature
2020年3月4日 · CRBN-knockout cells were obtained from OPM-2 and 293T cells by transfecting a pX330-based CRISPR/Cas9 vector 30 targeting the first exon of CRBN, and treating the cells with 0.1 µM CC-885 for 2...
Discovery of First-in-Class PROTAC Degraders of SARS-CoV-2 …
2024年4月12日 · MPD2 effectively reduced M Pro protein levels in 293T cells, relying on a time-dependent, CRBN-mediated, and proteasome-driven mechanism. Furthermore, MPD2 exhibited remarkable efficacy in diminishing M Pro protein levels in SARS-CoV-2-infected A549-ACE2 cells.
Degradation-Based Protein Profiling: A Case Study of Celastrol
2024年4月25日 · Flag-CRBN or Flag-DCAF16 lentivirus was generated by co-transfection of Flag-CRBN or Flag-DCAF16, psPAX2 and pMD2.G into 293T cells using Lipofectamine 3000 (L3000015). Virus-containing medium were collected 48 h after transfection, filtered with a 0.45 µm filter and used to transduce 293T cells.
Glutamine triggers acetylation-dependent degradation of …
2016年3月3日 · Cereblon (CRBN), a substrate receptor for the cullin–RING ubiquitin ligase 4 (CRL4) complex, is a direct protein target for thalidomide teratogenicity and antitumor activity of immunomodulatory drugs (IMiDs). Here we report that glutamine synthetase (GS) is an endogenous substrate of CRL4 CRBN.
CRBN 基因敲除细胞(293T)_源井生物【官网】 - ubigene.com
源井生物提供的CRBN 基因敲除细胞(293T)是基于红棉系统设计最优方案，采用超传统效率10-20倍的CRISPR-U™技术以及独家EZ-editor™系列产品构建而成，已进行Sanger测序法验证敲除并附STR鉴定报告，保证无支原体污染、活性良好，同时全程提供免费的培养技巧与技术 ...
Characterization of dWIZ-1 as a molecular glue degrader of WIZ
2024年7月4日 · We report the discovery of dWIZ-1 and dWIZ-2, molecular glue degraders of the WIZ transcription factor that robustly induce HbF in erythroblasts. Phenotypic screening of a cereblon (CRBN)–biased chemical library revealed WIZ …