2006年11月3日 · Lentivirus production: We have established a stable 293SF cell line (adapted to serum-free culture) expressing both the cumate repressor (CymR) and the reverse tetracycline …

2024年9月5日 · By utilizing both the Tet-On and cumate repressor systems in a single cell line, we generated a new system to investigate potential cooperation of PAX3::FOXO1 and HES3, …

2023年6月8日 · Lentiviral vectors (LVs) are important for cell therapy because of their capacity to stably modify the genome after integration. This study describes a novel and relatively simple …

2023年7月5日 · Here, we develop Tango-Trio, a comprehensive cell-based high-throughput platform comprising cumate-inducible expression of transducers, capable of the parallelized …

Here we report a novel strategy to illuminate cell proximity using transcriptional activators. We repurposed cumate, a small molecule inducer of gene expression, by caging its key …

这项研究描述了一种新颖且相对简单的方法，用于生成用水泡性口炎病毒糖蛋白（VSV-G）假型化的自失活（SIN）LV的包装细胞和生产者克隆。开发了一种基于 cumate 和香豆霉素诱导系统 …

We have developed a new inducible system for mammalian cells that can be used for applications that require tight control as well as those that require a high level of expression. We have …

2017年8月10日 · A CHO cell line containing the cumate gene switch elements (CHO Cum2) was transduced with lentiviral vectors for the generation of stable pools able to produce volumetric …

Lentiviral vectors (LVs) are important for cell therapy because of their capacity to stably modify the genome after integration. This study describes a novel and relatively simple approach to …

2006年11月3日 · Results: We have used the regulatory mechanisms of bacterial operons (cmt and cym) to regulate gene expression in mammalian cells using three different strategies. In …