2015年12月30日 · We set out to exploit FKBP DD-technology to control PTEN protein function in a rapid, reversible and tunable manner. FKBP is a 12kDa FK506 binding protein, which is …

The DD is a 12-kDa (107-amino-acid) tag based on a mutated FKBP protein. When a protein of interest is expressed as a fusion with the DD tag, it is destabilized and rapidly degraded in the...

2022年6月21日 · “不稳定结构域”（destabilizing domains，DD），是FK506-雷帕霉素结合蛋白FKBP突变体L106P，大小12kD。当它与其它蛋白融合后,会由泛素-蛋白酶体系统介导被快速 …

“不稳定结构域”（destabilizing domains，DD），是FK506-雷帕霉素结合蛋白FKBP突变体L106P，大小12kD。 当它与其它蛋白融合后,会由泛素-蛋白酶体系统介导被快速组成型降解。

We call these FKBP mutants destabilizing domains (DDs), because their instability is conferred to any protein fused to the DDs. A cell-permeable ligand binds tightly to the DDs and prevents …

2010年9月24日 · The ecDHFR-DD system successfully regulates transmembrane proteins that were difficult to target with the FKBP-DD, such as a GPCR protein, beta2-adrenergic receptor. …

迄今为止，已经描述了几种蛋白质/配体组合的 DD，例如 FKPB/Shield-1（雷帕霉素类似物）、26 DHFR/甲氧苄氨嘧啶、27 UnaG/胆红素28或人类雌激素受体结合域/他莫昔芬，29分别。多种 …

Shield-1 (Shld1) is a specific, cell-permeant and high-affinity ligand of FK506-binding protein-12 (FKBP), and reverses the instability by binding to mutated FKBP (mtFKBP), allowing …

2008年11月15日 · The FKBP-derived destabilizing domains are increasingly being used to confer small molecule-dependent stability to many different proteins. The L106P domain confers …

2015年12月30日 · Targeting protein stability with small molecules has emerged as an effective tool to control protein abundance in a fast, scalable and reversible manner. The technique …