2021年9月23日 · DEAD-box RNA (DDX) helicases are characterized by the presence of a specific amino acid motif, Asp-Glu-Ala-Asp (DEAD). DDX helicases comprise highly conserved core domains that regulate substrate binding, ATPase activity, and RNA unwinding helicase activity, though the catalytic activity may vary among DDX helicase [2, 3].

2024年8月30日 · DDX3X recognizes viral RNA and interacts with MAVS and TBK1, which phosphorylates IRF3/7 and DDX3; phosphorylated-DDX3 indirectly activates IFN-β transcription to enhance IFN production. The DDX1/DDX21/DHX36 complex binds to poly (I:C) dsRNA and activates IFN through the TRIF adaptor.

2018年9月26日 · Novel rG4 interactors included DDX3X, DDX5, DDX17, GRSF1 and NSUN5. The majority of identified proteins contained a glycine-arginine (GAR) domain and notably GAR-domain mutation in DDX3X and DDX17 abrogated rG4 binding.

Kevin Raney (University of Arkansas) described the binding of yeast Ded1 (human homolog DDX3X) to G-quadruplex (G4) DNA, leaking from the nucleus or mitochondria, initiating LLPS and stress...

2019年10月8日 · The DDX5 interaction with the MYC promoter and DDX5-mediated MYC activation is inhibited by G4-interactive small molecules. Our results uncover a function of DDX5 in resolving DNA and RNA G4s and suggest a molecular target to …

2024年3月26日 · Here, we show that inhibition of the RNA helicase DDX3 increased CD8 + T cell infiltration in syngeneic oral squamous cell carcinoma tumors. DDX3 knockdown compromised interferon-γ-induced PD-L1 expression and, in particular, reduced the level of cell-surface PD-L1.

2022年8月1日 · Methodological progresses and piling evidence prove the rG4 biology in vivo. rG4s step in virtually every aspect of RNA biology. Helicases unwinding of rG4s is a fine regulatory layer to the downstream processes and general cell homeostasis. The current knowledge is however limited to a few cell lines.

2019年9月18日 · DDX3X是一种酶，当发生突变时，参与多种癌症的产生，比如 乳腺癌 、肺癌和脑癌，包括成神经管细胞瘤，即一种最为常见的恶性儿童脑瘤。 DDX3X突变也与DDX3X综合征有关，这种综合征的特征是智力障碍、癫痫发作、自闭症、肌肉张力差和身体发育缓慢。 这些研究人员证实DDX3X也处于决定应激细胞（stressed cell，即遭受应激的细胞）生死的十字路口。 这种分子有助于调节先天免疫反应。 他们报道有证据表明DDX3X的可用性影响细胞如何理解和应对 …

2019年7月12日 · Here, we describe the structure of a D1D2 core in complex with a 23-base pair dsRNA at pre-unwound state, revealing that two DDXs recognize a 2-turn dsRNA, each DDX mainly recognizes a single RNA...

DEAD-box RNA (DDX) helicases are characterized by the presence of a specific amino acid motif, Asp-Glu-Ala-Asp (DEAD). DDX helicases comprise highly conserved core domains that regulate substrate binding, ATPase activity, and RNA unwinding helicase activity, though the catalytic activity may vary among DDX helicase [2, 3].