2024年7月11日 · Here, we demonstrated that the human ortholog of Dhh1p, DDX6, triggers the deadenylation-dependent decay of inefficiently translated mRNAs in human cells. DDX6 interacts with the ribosome through the Phe-Asp-Phe (FDF) motif in its RecA2 domain.

2024年2月6日 · The RNA helicase DDX6 is emerging as an important accessory protein for RISC function 27,28, and associates with RISC via direct binding to CNOT1 of the CCR4-NOT complex, which in turn binds...

2017年2月20日 · DDX6 is a conserved DEAD-box protein (DBP) that plays central roles in cytoplasmic RNA regulation, including processing body (P-body) assembly, mRNA decapping, and...

2023年2月6日 · We found that adenosine deaminases that act on RNA 1 (ADAR1) interact with DDX6 in the nucleus. Utilizing our newly developed dual-fluorescence reporter assay, we elucidated the DDX6 function as negative regulators in cellular ADAR1p110 and ADAR2.

2021年3月17日 · In this study, we investigated Ddx6, an essential component of processing bodies (PBs) that executes RNA decay and translational repression in the cytoplasm and participates in the cellular...

2018年10月1日 · The RO60/DDX6 axis regulates RNA decay for pre-existing RNAs at the early reprogramming stage. To determine whether RNA decay or translational repression occurs in miRNA-mediated RNA silencing, we measured the amount and stability of miR-302b-3p-targeted RNA expression using BRIC-qPCR .

The RNA-binding ability and RNA helicase activity of DDX6 contribute to its function in regulation of various pathways in RNA metabolism: processing body (P-body) formation, miRNA-mediated gene silencing, RNA decay, and translational repression [12,13,14,15].

2024年8月30日 · DDX helicases, a conserved group of RNA-binding proteins, regulate various cellular RNA processes, including ribosome biogenesis, translation, and splicing, which require recognition and processing of RNA structures (6, 11, 35, 60). Emerging research suggests their involvement in innate immunity by interacting with viral RNA structures and ...

Importantly, mutations that disrupt the DDX6-CNOT1 interaction impair miRISC-mediated gene silencing in human cells. Thus, CNOT1 facilitates recruitment of DDX6 to miRNA-targeted mRNAs, placing DDX6 as a downstream effector in the miRNA silencing pathway.

DDX6 (Rck/p54), a member of the DEAD-box family of helicases, is highly conserved from unicellular eukaryotes to vertebrates. Functions of DDX6 and its orthologs in dynamic ribonucleoproteins contribute to global and transcript-specific messenger RNA (mRNA) storage, translational repression, and dec …