2019年8月19日 · DFO was used to examine the role of iron in the contributions of FPN1 to cell growth and transformation. DFO reduced myeloma cell viability in a time-dependent manner, but not in a...

2018年5月29日 · Macrophages release iron into the bloodstream via a membrane-bound iron export protein, ferroportin (FPN). The hepatic iron-regulatory hormone hepcidin controls FPN internalization and...

Assessment of cytoplasmic hepcidin and ferroportin (FPN) protein levels in control and deferoxamine (DFO)-treated MCF-7 cells. A, Lysates of cells treated with 30, 100, or 300 µM DFO and those of controls were assessed for hepcidin and …

2008年11月16日 · Fpn-mediated iron loss induces ferritin degradation by the proteosome. In this study we show that permeable iron chelators, desferirax or deferriprone also induce the proteosomal degradation of ferritin. In contrast, DFO-mediated iron chelation at clinically useful concentrations, leads to ferritin degradation in lysosomes.

2025年1月15日 · Our findings suggest that the downregulation of FPN may contribute to the pathogenesis of RA, indicating a potential role of iron dysregulation in this disease, and FPN regulates the proliferation and migration of FLS by promoting the levels of ROS in FLS as well as suppressing the PI3K/AKT signaling pathway.

2018年7月20日 · Fifty millimolar DFO also increased Fpn. Together, these data point towards a transcriptional induction of Fpn in response to changes in cellular iron levels. Epigenetic regulation of Fpn may also occur as changes in genes associated with epigenetic regulation of …

2022年8月2日 · To investigate the effect of iron deficiency on hepatocyte functions, human healthy hepatocytes were treated with deferoxamine (DFO, 100 μM) to remove ∼30% of their total iron content, thus mimicking iron levels in human NAFLD/NASH hepatocytes (Figure S2 A). We assessed the impact of reduced iron content on the hepatocyte transcriptome.

Nerve growth factor-beta (NGF-β)-differentiated PC12 cells, used as a model of neuronal cells, were evaluated in terms of their viability and expression of ferroportin after inducing cellular iron overload with ferric ammonium citrate (FAC) or hepcidin, iron deficiency with deferoxamine (DFO), or hepcidin in combination with FAC or DFO.

2018年3月21日 · With regard to FPN expression, it significantly increased in cells treated with 300 µM DFO for 24 hours and those treated with 30, 100, or 300 µM for 48 hours; untreated cells showed minimal levels of FPN at either time point (Figure 3A and B).

2022年3月22日 · In this study, we established a rat model of CKD by 5/6 nephrectomy, treated CKD rats with the ferroptosis inducer, cisplatin (CDDP), and the ferroptosis inhibitor, deferoxamine mesylate (DFO),...