The RPMI-8402 human pre-T lymphoblastoid cell line was induced to cell death by DMSO. Analyses rescued at early times of treatment prove a substantial modification of plasma …

I first dissolved the drug in 100% DMSO to 25mM and then further diluted in RPMI to 10mM and 1mM respectively. I realised that the 10mM concentration formed a lot of crystals and will not...

After isolating PBMC with Ficoll-hypaque gradient, the cells are washed with RPMI containing penicillin/streptomycin twice. Freezing medium (90% FBS + 10% DMSO) is prepared before …

This protocol describes the freezing of PBMCs (with either 90% FBS/10% DMSO or 12.5% HSA in RPMI/10% DMSO) and the general thawing procedure with complete RMPI medium.

2021年1月1日 · To identify the optimum formulation of FBS and DMSO, we opted to test various concentrations of FBS. Three concentrations of 90%, 60%, and 30% FBS were included, the …

2013年1月1日 · DMSO was added to RPMI 1640 to achieve a concentration range of 0.125% to 4% (v/v). Of the 14 isolates tested, 10 (71%) demonstrated reduced growth when exposed to …

Results: Exposure of RPMI 8226 and Dox-40 myeloma cells to low concentrations of DMSO resulted in a marked increase in cell growth as detected by viable cell counts and cell …

1996年11月1日 · We demonstrate that dimethyl sulfoxide (DMSO) facilitated a better DNA uptake in four different cell lines (HL60, TR146, Cos-7 and L132). The cells were electroporated with …

Untreated and DMSO-treated human RPMI-8402 cells were observed using both morphological and biochemical methods, including measurement of membrane fluidity, intracellular Ca2+ …

2018年12月1日 · CD4 + T cells (10 6 per ml) were cultured in RPMI containing 5 mM glucose, 2 mM l-glutamine and 5% heat-inacitvated human male AB serum and DMSO. Cells were …