
Biophysical characterization of microbial rhodopsins with DSE motif
2023年3月3日 · To explore functionally novel microbial rhodopsins, we comprehensively checked motif residues in the microbial rhodopsins in microbiomes associated with terrestrial lichens and found a new group of 16 rhodopsins having “DSE motif”. They are phylogenetically distinct from other microbial rhodopsin groups ( Figure 1 ).
Similarities and differences of polyadenylation signals in human …
2006年7月12日 · Our refined DSE motif, a U-rich motif with conserved G residues, confirms the presence of a DSE in Drosophila, as suggested earlier in word-searches using a smaller set of ESTs . Its similarity with the human DSE is consistent with observations that crucial residues for RNA binding in C-terminus of the RNA-binding domain of CstF-64 are ...
Does the proposed DSE motif form the active center in the …
2002年10月2日 · This study tested the hypothesis that a DSE motif formed the active center of the Hermes transposase. All three residues, D402, S535, and E572 were mutated and the resulting mutated transposases were tested for activity using an in vivo inter-plasmid transposition assay performed in D. melanogaster embryos (Sarkar et al., 1997). While mutations ...
The seemingly identical 7SK and U6 core promoters depend on …
In addition to the core promoter, efficient expression of both genes in vivo requires the presence of a distal sequence element (DSE). At first, this DSE was considered to be identical in both genes, since in both cases octamer motifs are localized at …
A single base pair deletion from the inactive octamer-like motif of …
1991年12月15日 · Octamer sequence elements were analyzed for their capacity to induce the 7S K “core” promoter in vivo. The U6 distal sequence element (DSE) which contains a consensus sequence octamer, was able to support efficient 7S K expression in vivo.
Does the proposed DSE motif form the active center in the …
2002年10月2日 · Donor cleavage and strand transfer are two functions performed by transposases during transposition of class II transposable elements. Within transposable elements, the only active center described, to date, facilitating both functions, is the so-called DDE motif. A second motif, R-K-H/K-R-H/W-Y, is …
Biophysical characterization of microbial rhodopsins with DSE motif
2023年3月8日 · Here, we found microbial rhodopsins having a DSE motif in the third transmembrane helix from members of the Actinobacteria. Although the expressed proteins exhibited blue-green light absorption, either no or extremely …
Fine-Tuning the Function of Farnesene Synthases for Selective …
We found that the yield of β-farnesene was significantly reduced in the D471N variant, while it was maintained in the S475T variant , indicating that AaFS prefers the DSE and DTE motifs for Mg1 coordination.
Similarities and differences of polyadenylation signals in human …
2006年7月12日 · We demonstrate that the DSE is a specific motif in both human and Drosophila. These findings shed light on the sequence correlates of a highly conserved biological process, and improve in silico prediction of 3' mRNA cleavage and polyadenylation sites.
Biophysical characterization of microbial rhodopsins with DSE motif
To explore functionally novel microbial rhodopsins, we comprehensively checked motif residues in the microbial rhodopsins in microbiomes associated with terrestrial lichens and found a new group of 16 rhodopsins having “DSE motif”. They are phylogenetically distinct from other microbial rhodopsin groups (Figure 1).