2018年12月10日 · Here, we report that IDR acetylation/deacetylation regulates LLPS and assembly of stress granules (SGs), membrane-less organelles forming in response to stress. Acetylome analysis revealed that...

2024年11月5日 · Upon cellular stress, the IDRs of DDX3X (particularly its N-terminal IDR) drive it to undergo liquid-liquid phase separation (LLPS) and participate in stress granule formation, which leads to...

2024年2月1日 · In this Review, we summarize the current understanding on the relationship among DDX3X, LLPS, and neurodevelopmental disorders (NDDs) by providing a comprehensive analysis of DDX3X functions and mechanism of action, its roles in LLPS, and disturbances in this process caused by disease-related mutations.

2024年6月25日 · Mechanistic study shows that the SG protein DDX3X promotes cGAS phase separation and activation in a manner dependent on its intrinsic LLPS. USP8 cleaves K27-linked ubiquitin chains from the intrinsically disordered region (IDR) of …

The N-terminal IDR of DDX3X (IDR1) can undergo LLPS in vitro, and its acetylation at multiple lysine residues impairs the formation of liquid droplets. We also demonstrated that enhanced LLPS propensity through deacetylation of DDX3X-IDR1 by HDAC6 is necessary for SG maturation, but not initiation.

2025年2月6日 · The structure-function relationship of the IDRs in the catalytic cycle of DDX3X and its link to condensates remains to be established. However, our data indicate that the enzymatic functions of DDX3X are essential for regulating RNP granule assembly, while IDR-dependent DDX3X phase behavior may be less critical in complex RNP granule-like systems.

Mechanistic study shows that the SG protein DDX3X promotes cGAS phase separation and activation in a manner dependent on its intrinsic LLPS. USP8 cleaves K27-linked ubiquitin chains from the intrinsically disordered region (IDR) of DDX3X to enhance its condensation.

The N-terminal IDR of DDX3X (IDR1) can undergo LLPS in vitro, and its acetylation at multiple lysine residues impairs the formation of liquid droplets. Enhanced LLPS propensity through deacetylation of DDX3X-IDR1 by HDAC6 is necessary for SG maturation, but not initiation (PMID:30531905).

2024年6月11日 · The CYCT1 HRD potentiated four distinct C > U editors (V1, bt1-RESCUE, 6e-RESCUE, and CURE); potentiated V1 formed puncta; distinct IDRs (DDX4, DDX3X, HNRNPA1) were able to replace the CYCT1 HRD to enhance editing and induce puncta; and finally, mutations in DDX3X IDR that impaired LLPS also compromised its ability to enhance editing.

2022年5月27日 · 他们 发现DDX3Y蛋白的N端内在无序序列 （intrinsically disordered region， IDR） 相比于DDX3X的N端内在无序序列能更强的促进其液-液相分离；DDX3Y的腺苷三磷酸酶活性（ATPase）比DDX3X低，并且这一活性差异造成DDX3Y的液-液相分离聚集体更加缓慢的解聚；DDX3Y通过液-液相分离形成的聚集体比DDX3X更加严重的抑制蛋白翻译过程；DDX3Y通过液-液相分离形成的聚集体比DDX3X更加促进FUS蛋白的聚集。 总结起来， 本研究通过体外和细 …