DHE (Dihydroethidium) Assay Kit - Reactive Oxygen Species (ab236206) measures ROS directly in live cells. This kit uses DHE as a fluorescent probe for the detection of ROS generation and is specific for superoxide and hydrogen peroxide.

The superoxide indicator dihydroethdium, also called hydroethidine, exhibits blue-fluorescence in the cytosol until oxidized, where it intercalates within the cell's DNA, staining its nucleus a bright fluorescent red. Dihydtroethidium is also available in conveniently packaged 5 mM solution stabilized in DMSO (D23107). For Research Use Only.

碧云天研发生产的超氧阴离子活性氧检测试剂盒 (DHE) (Reactive Oxygen Species Assay Kit for Superoxide Anion with DHE)，简称ROS Assay Kit for Superoxide Anion with DHE，是一种以DHE为荧光探针，快速灵敏地检测细胞内超氧阴离子活性氧的试剂盒。 本试剂盒可使用荧光显微镜、激光共聚焦显微镜、流式细胞仪、荧光酶标仪等荧光检测系统进行检测。 活性氧 (Reactive oxygen species, ROS)主要包括超氧阴离子 (·O2-)、过氧化氢 (H2O2)、羟自由基 (·OH)等。

ROS induce damage in DNA, protein and lipids with important consequences in cells. Cell permeant reagent Dihydroethidium (DHE) is a fluorogenic dye that is useful for the detection of reactive oxygen species (ROS). DHE has been shown to be oxidized by superoxide to form 2-hydroxyethidium (2-OH-E+) (ex 500-

In this chapter, we describe a fluorescence dye-based detection method for assaying superoxide and mitochondrial superoxide in mouse aorta using dihydroethidium (DHE) and MitoSOX.

Jan 19, 2024 · This protocol provides an in-depth description of the various procedures involved in the assessment of ROS within the cells, including - preparation of DHE solution, incubation of cells with DHE solution, and measurement of DHE intensity necessary to …

Oct 1, 2016 · Reactive oxygen species burst induced by aluminum stress triggers mitochondria-dependent programmed cell death in peanut root tip cells.

Oxidative stress results from an imbalance in the production of reactive oxygen species (ROS) and the ability of the cell to scavenge them. ROS react with nucleic acids, proteins and lipids causing cell and tissue damage and can be measured using selective or general indicators.

ROS Detection Cell-Based Assay Kit (DHE): Measure superoxide and hydrogen peroxide levels in living cells. Synonym: Reactive Oxidation Species CBA Kit (dihydroethidium).

Dihydroethidium (hydroethidine or DHE) is a widely used ethidium-based, redox-sensitive fluorescent probe. DHE has been shown to be oxidized by superoxide to form 2-hydroxyethidium (2-OH-E+) (ex 500-530 nm/em 590-620 nm) or by non-specific oxidation by other sources of reactive oxygen species (ROS) to