2021年7月9日 · DIGE (双向荧光差异凝胶电泳,Two-dimensional fluorescence difference in gel electrophoresis,2D-DIGE)，是在传统双向电泳的基础上发展而来的新型蛋白质组学定量技术，其分离蛋白的基本过程与传统双向电泳一致。但DIGE使用三种荧光染料（Cy2, Cy3和Cy5）标记不同组别的蛋白，并在同一张凝胶上对标记好的三组蛋白混合物进行电泳分离，是一种多通道分离技术。 通常情况下，Cy3和Cy5分别标记两组蛋白，Cy2标记所有组别蛋白的混合物，作为内参使用 …

Difference gel electrophoresis (DIGE) is a form of gel electrophoresis where up to three different protein samples can be labeled with size-matched, charge-matched spectrally resolvable fluorescent dyes (for example Cy3, Cy5, Cy2) prior to two dimensional gel electrophoresis.

差异凝胶电泳技术( two-dimensional difference gel electrophoresis，2D-DIGE) 建立在双向电泳技术的基础上，能够在同一块双向电泳胶中分离多于一种品。 Jonathan 实验室最先使用这项技术，后来被Amersham Biosciences 公司进一步的优化并市场化。

DIGE facilitates the co-separation of two proteomes to be compared, thereby diminishing the number of gels to be processed, evaluated, and interpreted by a factor of two. This saves critical resources like time and manpower.

Two-dimensional difference gel electrophoresis (2D-DIGE) remains to be one of the most popular and versatile methods of protein separation among many proteomics technologies. Similar to traditional two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the proteins are separated based on their charges and molecular weight by 2D-DIGE.

2020年1月28日 · Two-dimensional difference gel electrophoresis (2D-DIGE) remains to be one of the most popular and versatile methods of protein separation among many proteomics technologies. Similar to traditional two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the proteins are separated based on their charges and molecular weight by 2D-DIGE.

2012年2月13日 · 采用dige技术，通过对不同类型，不同个体的细胞、组织、或经过不同处理和不同生长条件下蛋白质表达差异分析，在研究疾病的分子机理、分子诊断、药物作用机理、毒理学等方面都有广泛的应用。

双向差异凝胶电泳（two dimension difference gel electrophoresis, 2D-DIGE）是一种新出现的 荧光 标记的 定量蛋白质组学 技术，比经典的2-DE具有更高的 动力学 范围和灵敏性。 DIGE系统基于荧光差异 凝胶电泳。 （2D.DIGE）技术，是利用电荷和分子量的 差异分离 蛋白质混合物，并通过多通道激光扫描分析不同蛋白质的第二向SDS-PAGE凝胶图像。 DIGE系统结合了新型 荧光技术 、样品多路技术（sample multiplexing）和 图像分析 等特有的技术，是一套优于经典的2-DE的 …

2006年9月1日 · Difference gel electrophoresis coupled with mass spectrometry (DIGE/MS) is a technology used within proteomics that has demonstrated technical robustness and associated statistical confidence to enable successful identification of therapeutic targets.

The two-dimensional difference gel electrophoresis method is a valuable approach for proteomics. The method, using cyanine fluorescent dyes, allows the co-migration of multiple protein samples in the same gel and their simultaneous detection, …