2023年8月9日 · 共同伴侣辅助蛋白（DnaJ/Hsp40）刺激ATP水解为ADP（黄色），导致底物结合域发生巨大的构象变化，导致其构象闭合，底物被锁定在底物结合域；这里促进了适当的折 …

2012年3月9日 · Upon deletion of TF, DnaK interacts increasingly with ribosomal and other small, basic proteins, while its association with large multidomain proteins is reduced. DnaK also …

The 70 kilodalton heat shock proteins (Hsp70s or DnaK) are a family of conserved ubiquitously expressed heat shock proteins. Proteins with similar structure exist in virtually all living …

2012年3月29日 · DnaK, the major bacterial Hsp70, is one of the most abundant constitutively expressed and stress inducible chaperones in the E. coli cytosol. Yet it is not essential under …

2020年1月17日 · The ATP-dependent Hsp70 chaperones (DnaK in E. coli) mediate protein folding in cooperation with J proteins and nucleotide exchange factors (E. coli DnaJ and GrpE, …

DnaJ proteins are molecular co-chaperones that recruit DnaK family chaperones (heat shock family) to perform functions such as protein folding, protein transport, or remodeling of protein …

2024年1月22日 · Here we report a cryo-EM analysis of GrpE bound to an intact Mtb DnaK, revealing an asymmetric 1:2 DnaK−GrpE complex. The GrpE dimer ratchets to modulate both …

2022年6月16日 · Our results demonstrate that the initial DnaK response is largely dependent on protein synthesis rate but as the recombinantly expressed protein accumulates and …

We investigated the interaction of the major inducible hsp from mammalian (Hsp70) and bacterial (DnaK) species with liposomes. We found that mammalian Hsp70 displayed a high specificity …

来自大肠杆菌的热休克蛋白DnaK，DnaJ和GrpE（KJE）构成三组分分子伴侣系统，该分子伴侣系统可防止变性蛋白质聚集并以ATP依赖性方式协助蛋白质重新折叠。