Definitely Not a Void (DNaV) is a nil difficulty, mixed-progression based, wacky frame DNaC that was to be located in Ring 1, removed, and readded to Realm 1. This DNaC is the second hardest NEaT in the game, behind The Neat Centurial.

DNaV is a well-known nil difficulty DNaC, with a 10-floor ascension section, 4-floor sideways progression, and concluding with an 8-floor descension section. DNaV is no longer found in Ring 1 and is now found in Realm 1. The current difficulty range of this DNaC's floors ranges from Challenging to nil.

Game Updates Ring 3 has been revamped. We are changing the location of TN and DNaV to fit JToH's new win rooms.

Definitely Not a Void (or DNaV for short) is a Nil wacky frame DNaC in Ring 1. It is a community-based DNaC made by bigeengle. This should be attempted after every single floor on TNP:R. It is based off of Citadel of Void.

2019年7月12日 · ROBLOX TNP | How to get in DNaVAlfie Alvarez (Blueguest450)Easy way

Go to where you would find DNaV's portal, but instead, go to the custom realms section. Wall hop on the neon outline, and at the top, there should be a hard to see ladder. Climb it, go inside the room, and you will see TNC's portal. Go in the portal, then you should be teleported to a tiny island with its actual portal there.

2006年5月22日 · Here, we discuss non-specific DNA-Tn5 Transposase (Tnp) interactions and suggest how they stabilize the Tnp and modulate Tnp localization of the 19 bp Tnp recognition end sequences (ESes). DNA protection assays indicate that full-length Tnp interacts efficiently with supercoiled DNA that does not contain ESes.

2018年2月9日 · IS50L和IS50R均含有编码转座酶（TnP）以及转座阻遏蛋白（lnh）的基因，但由于IS50L中的碱基突变，造成翻译提前终止，所以仅有IS50R可以产生正常的有活性的TnP和lnh。

2022年4月12日 · 研究发现，Tn5转座酶可以仅识别OE序列在DNA中随机插入序列，完成体外的转座。 根据这一原理，将测序的read1和read2加入OE序列中，Tnp识别并剪切含有OE序列的read1和read2序列后便可形成Tn5转座复合体。 其中灰色的序列即为19bp的OE序列（也称为 Mosaic End、MS序列）；蓝色：Nextera tn5 read1 (5’-TCGTCGGCAGCGTC-3’)；红色：Nextera tn5 read2 (5’-GTCTCGTGGGCTCGG-3’)。 也有的Tn5 library preparation kit的read1和read2 …

综上所述，该研究从ISDra2系统中 鉴定了一个新的具有dsDNA切割功能的核酸酶TnpB，其在原核和真核细胞中均能有效切割dsDNA，具有编辑人类基因组的巨大潜力。 在进化树上，虽然TnpB与微型 Cas12f 核酸酶的关系最为紧密，但作者认为两者之间依旧存在重大区别：（1）TnpB与Cas12f使用的guide RNA不同；（2）TnpB是单体，仅需要一个reRNA；而Cas12f 核酸酶是二聚体，需要结合一个拷贝的crRNA-tracrRNA duplex；（3）TnpB需要TAM序列，Cas12f需要PAM …