2024年6月12日 · Allogeneic double-negative T cells (DNTs) are a rare T-cell subset that effectively target acute myeloid leukemia (AML) without inducing graft-versus-host disease in an allogeneic setting. A phase 1 clinical trial demonstrated the feasibility, safety, and potential efficacy of allogeneic DNT therapy among patients with relapsed AML.

2018年4月24日 · We also observe complementary activity between conventional chemotherapy and DNT therapy on CD34 + primary AML samples, which suggests that DNTs can target chemotherapy-resistant cells in a clinical setting, especially after DNR treatment. Overall, the results of this study support the use of DNTs as an adjuvant cellular therapy following ...

Methods and results: Forty-one DNTs classified as three histological forms were investigated with CD34 and microtubule-associated protein 2 (MAP2) immunohistochemistry. CD34 immunoreactivity was more frequently observed in non-specific DNT types (16/18 cases; 88.9%) than in classic types (6/23 cases; 26.1%) (P < 0.001).

2021年4月22日 · Dead cells were identified as the CD3 − /Annexin V + population. For patient leukemic blasts, cells were stained with CD45, CD33, CD34, CD3, and CD64 to identify leukemic blasts with or without CD64 expression. The percentage of …

2011年5月13日 · These data demonstrate that ex vivo expanded DNT cells from AML patients can kill primary CD34 + leukemic blasts in vitro and that both DNT cell subsets contribute to this anti-tumor effect...

In this study, we explored a non-conventional T cell subset named double negative T cells (DNTs) as a novel therapy to target AML, including leukemic cells that are resistant to the conventional chemotherapy. DNTs are mature peripheral T lymphocytes expressing CD3-T cell receptor (TCR) complex but not CD4, CD8, nor invariant NK T cell markers.

2011年8月26日 · CD34 and microtubule-associated protein 2 expression in dysembryoplastic neuroepithelial tumours with an emphasis on dual expression in non-specific types. Aims: Three histological variants of dysembryoplastic neuroepithelial tumour (DNT) have been described, namely, simple, complex and non-specific. However, the concept of non-specific ...

2021年7月22日 · DNTs were cocultured with target cells for 2 to 4 hours, and cells were then stained with anti-human CD3 (HIT3a), CD33 (WM53), CD45 (HI30), and CD34 (561) antibodies, Annexin V, and 7AAD (all from BioLegend), and analyzed by using flow cytometry.

Methods: Immunohistochemical study for CD34 was performed on formalin-fixed paraffin-embedded tissue blocks of 54 cases of brain tumors occurring in patients with refractory epilepsy. The tumor types included ganglioglioma (GG, number = 21), dysembryoplastic neuroepithelial tumor (DNT, number = 8), tumors/lesions which had the transitional ...

DNT include the specific form and the controversial non-specific form that lack the specific glioneuronal element. Our aims were to search for BRAF(V600E) mutation and CD34 expression in DNT, PXA, GG and PA to correlate BRAF(V600E) mutation with BRAF(V600E) expression and to evaluate their diagnostic and prognostic values.